摘要
目的：建立植物油中黄曲霉毒素B1含量的液相质谱-串联质谱测定方法。方法：采用液相色谱-串联质谱法（LC—MS／MS），以乙腈为溶剂提取植物油中黄曲霉毒素B1。ZORBAX SB—C18（2．1mm×50mm，1．8μm）色谱柱，流动相为乙腈－0．2％甲酸（40：60）梯度洗脱，流速为0．3mL/min，进样量为5μL。采用电喷雾离子化四极杆串联质谱，多反应监测方式测定样品的浓度。检测离子对分别为m/z313．0→285．0和m／z313．0→241．0。结果：黄曲霉毒素B1进样量在0．204～4．08ng／mL（r=0．9991）范围内呈良好的线性关系，平均加样回收率为98．15％，RSD=1．12％（n=9）。结论：该法简便快捷、结果准确，可用于植物油中黄曲霉毒素B1的含量的测定。
Abstract：Objective：To establish an LC-MS/MS method to determine the content of Aflatoxin B1 in vegetable oil. Methods：Aflatoxin B~ was extracted from vegetable oil by acetonitrile. The analysis was performed on a ZORBAX SB-C18 column. The mobile phase consisted of acetonitrile-0.2% methane acid solution for gradient elution. The flow rate was 0.3mL/min. Then 5μL was injected for analysis, Electrospray ionization （ESI） sources was applied and operated in the position ion mode. Multiple reaction monitoring（MRM） mode with the transition of m/z 313.0→285.0 and m/z 313.0→240.0 were used to quantify Aflatoxin B1. Results.The sample was in good linear at the range of 0.204-4.08ng/mL（r=0.9991 ）. The average recovery rate was 98.15%（n=9）. The RSD was 1.12%. Conclusion ：The method was simple,quick and accurate,proved to be applyable in the content determination of Aflatoxin B1 in vegetable oil.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第18期72-74,共3页
Science and Technology of Food Industry
