摘要
目的 建立妇安消疹洗液中蛇床子素的含量测定方法,完善质量控制体系.方法 使用高效液相色谱法,色谱柱：Diamonsil-C18柱（250 mm ×4.6 mm,5μm）；流动相：乙腈-水（65：35）,检测波长：322 nm,流速：1.0 mL/min,进样量：10 μL,柱温：30℃.结果 蛇床子素在2.0～64.0 μg/mL范围内线性关系良好（r=0.999 8）,平均回收率为97.1％,RSD为1.4％（n=6）.结论 本方法简便快捷,专属性强,适用于妇安消疹洗液中蛇床子素的含量测定.
Objective To establish a method for the determination of osthole in fuanxiaozhen cleaning solution,and to provide a base for its quality criteria.Methods HPLC method was used.The C18 column（250 mm × 4.6 mm,5 μm）was used with the mobile phase of acetonitrile-water（65：35）.The detective wavelength was 322 nm,with the flow rate of 1.0 mL/min.The sample size was 10 μL,and the column temperature was 30 ℃.Results The osthole had a linear relationship in the range of 2.0 ～ 64.0 μg/mL （r =0.999 8）.The average recovery rate was 97.1％ （RSD =1.4％,n =6）.Conclusion The method is simple,sensitive,accurate and suitable for the determination of fuanxiaozhen cleaning solution.
出处
《实用药物与临床》
CAS
2013年第8期718-720,共3页
Practical Pharmacy and Clinical Remedies
基金
湖北省重大科学技术鉴定成果(EK2012D 150018000359)
太和科研基金资助项目(2009D34)
