摘要
呼吸气体携带重要的人体生理/病理状态的信息,且具有非侵入性、无创、方便、连续可得等特点,受到相关研究人员越来越多的重视。呼吸气体的检测在代谢组学、临床医学、安全卫生等领域中具有重要意义。同传统的色谱质谱检测技术相比,直接质谱分析技术在呼吸气体的检测中具有高灵敏度、高选择性、低损耗的特点,真正达到了实时在线、原位活体分析的要求。本文介绍了质子转移反应质谱(PTR-MS)、选择离子流动管质谱(SIFT-MS)以及电喷雾萃取电离质谱(EESI-MS)三种直接质谱分析方法的原理及其在呼吸气体检测中的应用实例,并展望了呼吸气体检测的应用前景和发展方向。
Breathing gas is receiving more and more attentions from researchers for the important physiological/ pathological information it carries,and its non-invasive,conveniently and continuously available nature.Detection of breathing gas is very important in studying the mechanism of human metabolism,clinical medicine,health and safety.Compared to traditional chromatography-mass spectrometry detection,direct mass spectrometric analysis for detecting breathing gas is a real-time,online and in-vivo technique with high sensitivity and low-loss requirements.In this article,we reviewed three kinds of direct mass spectrometry techniques：proton transfer reaction mass spectrometry（PTR-MS）,selected ion flow tube mass spectrometry（SIFT-MS）,and the extractive electrospray ionization mass spectrometry（EESI-MS） with their applications breathing gas detection.We also discussed the prospects and development of breathing gas detection.
出处
《现代科学仪器》
2013年第4期40-45,共6页
Modern Scientific Instruments
基金
江西省科技支撑计划项目(2010BSA10200)
国家质检总局科技项目(2011IK248)
浙江省科技厅公益项目(2011C23120)资助
