摘要
目的:探讨活性维生素D3对糖尿病大鼠肾脏骨形成蛋白-7(BMP-7)和转化生长因子-β1(TGF-β1)表达的影响。方法:30只雄性SD大鼠,按随机数字表法分3组:正常对照(NC)组,糖尿病模型(DM)组,活性维生素D3(DC)组(制备糖尿病模型后用骨化三醇灌胃),每组10只。12周后,检测大鼠24 h尿蛋白定量及血生化指标,采用PAS及Masson染色观察大鼠肾脏病理改变,免疫组化法检测肾组织中BMP-7及TGF-β1蛋白的表达,RT-PCR法检测肾组织中BMP-7、TGF-β1 mRNA的表达。结果:与NC组比较,DM组大鼠肾脏肥大指数、24 h尿蛋白定量、血肌酐、尿素氮、胱抑素C水平均明显升高(P<0.05);同时肾组织中TGF-β1 mRNA及蛋白表达水平也明显升高(P<0.05),而BMP-7 mRNA及蛋白表达水平明显下降(P<0.05)。与DM组比较,DC组肾组织中BMP-7 mRNA及蛋白表达水平明显升高(P<0.05),余上述指标明显下降(P<0.05),病理改变明显减轻。结论:活性维生素D3可能通过上调BMP-7、下调TGF-β1的表达,发挥其肾脏保护作用。
Aim：To explore the effect of 1, 25-dihydroxyvitamin D 3 on the expressions of bone morphogenetic protein-7（BMP-7） and transforming growth factor-beta 1（TGF-β1） in renal tissues of diabetic rats.Methods：A total of 30 SD rats were randomized into 3 groups： normal control （ NC） group, diabetic model （ DM） group and diabetic model treated with 1, 25-dihydroxyvitamin D3 （DC） group,and 10 in each group.After 12 weeks of treatment, the renal function, 24 hours total urine protein , blood calcium and so on were detected , PAS and Masson staining were performed to observe histo-logical damage in the kidneys , and immunohistochemical staining and RT-PCR were used to monitor the expressions of BMP-7 and TGF-β1 protein and mRNA in renal tissue.Results： Compared with those of NC group, kidney index,24 hours total urine protein, Scr, serum BUN and Cys-C levels were significantly higher in DM group（P〈0.05）,and the mR-NA and protein expressions of TGF-β1 in renal tissue were also higher（P〈0.05）,but the expressions of BMP-7 were lower （P〈0.05）.Compared with those of DM group, the indexes mentioned above improved significantly （P〈0.05）,and the histological damage was alleviated .Conclusion：1, 25-dihydroxyvitamin D3 has some renal protective effect on diabetic rats, partly through up-regulating BMP-7 and down-regulating TGF-β1 expressions in renal tissue .
出处
《郑州大学学报（医学版）》
CAS
北大核心
2013年第5期616-620,共5页
Journal of Zhengzhou University(Medical Sciences)
