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IFA和IPMA方法测定猪瘟兔化弱毒病毒含量 被引量:12

IFA and IPMA for HCLV Titer Detection
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摘要 使用间接免疫荧光方法(IFA)和过氧化物酶单层试验(IPMA)测定猪瘟兔化弱毒的病毒含量(TCID50)。两种方法都使用韩国JBT公司猪瘟单抗作为一抗,此单抗的稀释倍数最高为300倍。Trueblue过氧化物酶底物显色的、感染猪瘟兔化弱毒的阳性细胞呈现蓝色,易于辨识,所以确定Trueblue为IPMA方法中的酶作用底物。建立的的IFA和IPMA方法与兔体反应热法进行了比较,证明两者存在一定平行关系,但IFA和IPMA比兔体反应热法检测结果的数值低1个数量级左右。基于以上结果,TCID50方法可作为猪瘟疫苗效力检验的候选方法。 Indirect immunofluorescent assay (IFA)and Immunoperoxidase monolayer assay(IPMA) were used to titrate the Tissue Culture Infectious Dose 50(TCID50) of Hog cholera virus lapinized Chinese strain(HCLV). Optimized antibody dilution and Peroxidase Substrate. IFA and IPMA can titer TCID50 of finished and semi-finished products of HCLV vaccines. Though HCLV vaccines were tittered by RID and PD50, we could also titer TCID50 to make sure about vaccines quality . #$NLKeywords: hog cholera lapinized virus ; TCID50;IPMA;IFA
出处 《中国兽药杂志》 2013年第10期35-38,共4页 Chinese Journal of Veterinary Drug
关键词 猪瘟兔化弱毒 TCID50 IPMA IFA hog cholera lapinized virus TCID50 IPMA IFA
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参考文献5

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