摘要
从孤岛油田石油污染土壤中分离到一株高效石油降解菌,命名为SKD-1。该菌株菌落表面湿润光滑、边缘整齐、圆形、不透明、乳黄色,能够利用葡萄糖和淀粉作为其生长的碳源和能源,其最适生长环境为碱性(pH8-10),在分别以正十六烷烃和原油为惟一碳源,温度为30℃,摇床(180r/min)培养的条件下,菌株SKD-1的降解率分别为66.1%和36.9%。16SrRNA基因序列分析表明,菌株SKD-1与不动杆菌AcinetobactercalcoaceticusSY-1同源性达99%。结合菌株SKD.1菌落形态、理化性质以及系统发育分析,可以鉴定菌株SKD-1属于不动杆菌属(Acinetobactersp.),序列登录号为AB774229。
A strain named SKD-1 was isolated from Gudao oil field. The colonial morphology of this bacte- ria is wet-smooth, round, opacity and cream-yellow. Glucose and starch could be utilized as the carbon and en- ergy source by this bacteria. The optimum growth environment for strain SKD-1 was alkalinity. Its degrading rate of hydrocarbon reached 66. 1% and 36.9% respectively under the culturing conditions of 30℃ with shaking (180 r/min) , 16 paraffin or crude oil as the sole carbon sources in the medium. 16S rRNA sequence analysis showed a high similarity of 99% with the most related strain Acinetobacter calcoaceticus (X81661) , based its colo- nial morphology, physiochemical properties and phylogenetic tree results, strain SKD-1 was regarded to be one member of Acinetobacter genus and named Acinetobacter SKD-1 (AB774229).
出处
《环境工程学报》
CAS
CSCD
北大核心
2013年第10期4116-4120,共5页
Chinese Journal of Environmental Engineering
基金
山东省博士后创新项目专项基金一等资助(201201008)
关键词
石油污染
高效降解菌降
解率
petroleum contamination
high-efficient degrading bacteria
degradation rate