摘要
目的研究婴幼儿病毒性腹泻的病原构成,为该病的防治提供科学依据。方法采用流行病学的调查方法对病人进行调查;采用ELISA法和RT-PCA法检测病毒病原。结果病毒学检测结果:416份腹泻样本中,阳性样本222份,阳性率为53.37%,其中轮状病毒(HRV)阳性170份(占40.87%),杯状病毒(HuCV)阳性样本29份(占6.97%),星状病毒(AstV)阳性样本4份(占0.96%),腺病毒(AdeV)阳性样本19份(占4.57%),各种混合感染34例(占8.17%);HRV感染发病,以每年的12月份和次年的1月份为发病高峰;HuCV感染发病,以每年的11、12月份为发病的高峰;AdeV感染发病,以每年的12、6月份为发病高峰;Astv感染发病,以每年的1、2月份为发病高峰。HRV、HuCV、AdeV、Astv患儿的月龄分布:均以12~月龄组最多,分别为45.23%、38.29%、43.75%、29.41%。轮状病毒的G3血清型为流行毒株(66.67%)G9血清型大幅度上升(23.58%)P基因型分型结果:以P[8]为主(68.13%),其次是P[4](20.88%)。结论婴幼儿腹泻病毒病原主要是HRV,其次是HuCV、AdeV、Astv,HRV血清型G3和基因型P[8]、P[4]是主要的流行株,其次是很少见的血清型G9,这一结果为HRV疫苗研究提供重要信息。
Objective To study on etiology of diarrhea among children and to provide the scientific basis for the pre- vention of the virus diarrhea disease. Method Using epidemiological method to survey patients with diarrhea,virus was detected by enzyme linked immuno assy (ELISA) and revere-transcription polymeras chain reaction (RT-PCR). Results Virological testing results showed: the rate positive of virus was 53. 37%(222/416),40. 87% of Rotavirus(170/416), 6.97% of human caliciviruses (29/416) ,0.96% of Astrovirus (4/416) ,4. 57% of Adenovirus (19/416). 8. 17% of Mixed infection (34/416). The distribution of season: HRV infected highest was December and follow year of January. HuCV in fected highest was November and December. AdeV infected highest was December and June. Astv infected highest was Janu- ary and February. The distribution of month old of Virus diarrhea: HRV, HuCV, AdeV, AstV of infection most was 12% month groups (45.23%,38.29%,43.75%,29.41%). 66.67% of HRVG3 and p[8](68.13%),p[4](20.88%) were most common stain. 23.58%of HRV G9 serotype rose significantly. Conclusions HRV is mainly pathogen of virus diarrhea in children, follow HuCV, AdeV, Astv. G3 and P[8], P[4] of the HRV are epdmics trains, the follow is Gg. These results pro vide important information for HRV vaccine research.
出处
《中国儿童保健杂志》
CAS
北大核心
2013年第10期1032-1034,共3页
Chinese Journal of Child Health Care
基金
国家"863"项目(2001AA2171)