摘要
使用砷钼酸比色法,以葡萄糖为标准品,在波长560nm处测吸光度值求得粗多糖含量。葡萄糖含量在0.02mg-0.10mg范围内与吸光度值呈线性关系,回归方程Y=5.845X-0.072 1,相关系数r=0.999 3,平均回收率为96.42%,相对标准差RSD=1.565%(n=5)。砷钼酸比色法方法易于操作,反应产物稳定,测试结果准确度高、重现性好,是测定保健酒及其它保健食品中粗多糖含量的理想方法。
By using the arsenomolybdate colofimetric method, with the glucose as a standard, the content of crude polysaccharides was obtained by measuring the absorbance at wavelength of 560 nm, In the range of 0.02mg - 0.10rag, the content of crude polysac- charides shows a linear relationship with its absorbance values. The fitted equation is Y = 5. 845X -0. 072 1, with a correlation coefficient r = 0.999 3 and an average recovery rate is 96.42% and the relative standard deviation RSD = 1. 565% （ n = 5）. This approach has advantages such as： easily handling, the product is stable, higllly precise, good reproducibility, so it can be an ideal method to measure the crude polysaccharides in the health wine and other health foods.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
北大核心
2013年第4期172-174,共3页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
关键词
砷钼酸比色法
保健酒
粗多糖
分光光度计
Arsenomolybdate colorimetric method
health wine
crude polysaccharides
spectrometer
