摘要
目的:探讨内皮生长因子-C(vascular endothelial growth factor-C,VEGF-C)、黏附分子CD24在肺癌组织中的表达及其临床意义。方法:采用RT-PCR和免疫组化方法检测138例原发性肺癌患者肿瘤组织及癌旁组织中VEGF-C、CD24的表达水平。结果:肺癌组织中VEGF-C、CD24 mRNA及蛋白的表达均高于癌旁组织(P<0.05),两者在肺癌组织中的表达明显正相关(P<0.05);有淋巴结转移组中VEGF-C、CD24 mRNA及蛋白的阳性表达量均高于无淋巴结转移组(P<0.05);VEGF-C、CD24表达与淋巴结转移、肿瘤TNM分期等临床病理特征间有明显相关性(P<0.05);VEGF-C、CD24蛋白表达与ⅢA期患者的短期预后有关,两者的mRNA水平与无病生存时间呈负相关。结论:VEGF-C、CD24在肺癌组织中均异常表达,可作为肺癌诊断的辅助标志物。
Objective： To study the expressions of vascular endothelial growth factor -C （VEGF-C） and CD24 in lung cancer and their clinical significance, and to discuss the relationship between their expressions as well. Methods： The methods of RT-PCR and im- munohistochemical staining are adopted. The expression levels of VEGF-C, CD24 mRNAs and proteins in 138 primary lung cancer pa- tients 7cancer tissues and paracancerous tissues are detected. Results： The expression levels of VEGF-C CD24 mRNA and proteins in lung cancertissues are all higherthanthosein paracaneeroustissues （P〈0.05）. Thereis evident positive eorrelativity between the expressions of VEGF-C and CD24 in lung cancer （P〈0.05）. The expression levels of VEGF-C, CD24 mRNA and proteins in themetastasis group are all higherthanthoseinthe non-metastasisgroup （P〈0.05）. Thereis evident correlative-ty between the expression of VEGF-C, CD24 and lymph node metastasis, TNM staging（P〈0.05）. The expressions of VEGF-C, CD24 mRNA and proteins are both related to the short-term prognosis of the Stage IlIApatients. Conclusion： VEGF-C and CD24 are both abnormally expressed in lung cancer tissues, which can be used as a marker of lung cancer diagnosis.
出处
《现代生物医学进展》
CAS
2013年第25期4908-4912,共5页
Progress in Modern Biomedicine
