摘要
目的:建立测定小儿清热止咳口服液中(R,S)-告依春含量的方法。方法:采用高效液相色谱法。色谱柱为ZORBAX SB-C18,流动相为乙腈-水-磷酸-三乙胺(9∶90∶0.95∶0.05,V/V/V/V),流速为0.7 ml/min,检测波长为245 nm,柱温为30℃,进样量为10μl。结果:(R,S)-告依春检测质量浓度在15.12～35.76μg/ml范围内与峰面积积分值呈良好的线性关系(r=0.999 0);精密度、稳定性、重复性试验的RSD≤1.0%;平均加样回收率为98.90%,RSD=1.06%(n=6)。结论:该方法回收率、精密度和稳定性均较理想,适用于小儿清热止咳口服液的质量控制。
OBJECTIVE： To establish the method for the content determination of （R, S）-Goitrin in Xiao＇er qingre zhike oral liquid. METHODS： HPLC was employed. The determination was performed on ZORBAX SB-C18 column with mobile phase con- sisted of acetonitrile-water-phosphoric acid-triethylamine （9 ： 90 ： 0.95 ： 0.05, V/V/V/V） at the flow rate of 0.7 ml/min. The detection wavelength was set at 245 nm and colunm temperature was 30 ℃. The injection volume was 10 μl. RESULTS： The linear range of （R,S）-Goitrin were 15.12-35.76 μg/ml （r=0.999 0） with an average recovery of 98.90% （RSD=1.06%, n=6）. RSDs of preci- sion, stability and reproducibility tests were all lower than 1.0%. CONCLUSIONS： The recovery, precision and stability of the method is optimal and suitable for the content determination of Xiao＇er qingre zhike oral liquid.
出处
《中国药房》
CAS
CSCD
2013年第40期3825-3827,共3页
China Pharmacy
