摘要
参照GenBank上已发表的牛病毒性腹泻病毒(BVDV)和猪瘟病毒(CSFV)的全基因序列,针对瘟病毒高度保守的5'-UTR设计3条引物,特异性扩增BVDV、CSFV。经过条件优化后,建立了快速鉴别BVDV和CSFV的双重RT-PCR诊断方法,扩增两种病毒的片段,大小分别为260、200 bp。试验证明,所建立的方法具有良好的特异性和敏感性,利用双重RT-PCR对临床上60份疑似病料进行检测,双重PCR检测的结果与单重PCR检测结果总体符合率为100%,可用于临床病料检测,为防止猪瘟细胞苗的污染及进行CSFV和BVDV的鉴别诊断提供了有效方法。
According to the complete genome sequences of bovine viral diarrhea virus （BVDV） and classical swine fever virus （CSFV） in GenBank, 3 primers were designed and synthesized. A double RT-PCR was established based on these 3 primers, and amplified the BVDV virus-specific segment with 260 bp and the CSFV virus-specific segment with 200 bp under the optimal conditions. To evaluate the double PCR, 60 clinical samples were detected. The data showed that the double PCR method was coincidence with single PCRs. The experiment showed that double RT-PCR method had good specificity and sensitivity of cell vaccine to prevent the pollution of BVDV and provide an effective method for detecting the diagnosis of CSFV and BVDV.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第19期131-133,共3页
Guangdong Agricultural Sciences
基金
福建省自然科学基金(2011J01233)
福建省教育厅A类科技项目(JA12315)
