摘要
目的：建立复方制剂护肝宁丸中的有效成分虎杖苷的含量测定方法。方法：HPLC法。色谱条件：色谱柱ZORBAXSB-C18（4．6×250nm5μm），以乙腈-水（20：80）为流动相；检测波长：306nm；流速：1．0ml／min。结果：虎杖苷在0．1692-1．269μg的范围内与峰面积呈良好的线性关系，r=0．9996，平均回收率为98．29％，RSD为0．79％。结论：本测定方法简便可行，重复性好，可用于本制剂中有效成分虎杖苷的含量测定。
Objective： To determine the concents of baicalin in polydatin in Huganning Pills. Methods： The HPLC method was established for the determination of polydatin in Huganning pills. The column is ZORBAX SB-C18 （4.6 × 250mm 5μm）, mobile phase consisting of acetonitrile-water （20 ; 80）, and the flow rare was 1.0ml/min, the detection wavelength is 306nm. Results： A better Liner relation was obtained form 0.1692 to 1.2691xg of polydatin for plasma with a good correlation coefficient; r=0.9996, the average recovery of ephedrine hydrochloride was 98.29%, RSD was 0.79%, the method has good precision repeatability. Conclusion： This method is simple, and quick.it can be used as quality control in Huganning pills.
出处
《中医临床研究》
2013年第19期27-28,共2页
Clinical Journal Of Chinese Medicine
