摘要
目的测定生血宝颗粒中何首乌苷（2，3，5，4’-四羟基二苯乙烯-2-O-β-D葡萄糖苷）的含量。方法采用高效液相色谱法，色谱柱为AgilentHC—C18（4．6mm×250mm×5μm）；流动相为乙腈一水（18：82）；流速为1．0ml／min，检测波长为320nm。结果何首乌苷进样量在3．580～114．5μg／ml范围内与峰面积呈良好的线性关系（r=0．99994，n=6），RSD=1．3％；平均回收率为100．3％，RSD=1．76％。结论高效液相色谱法简便易行，结果可靠，可有效地控制生血宝颗粒的质量。
Objective To establish a method for determining the content of Polygonum Multiflorum Gly- coside （2,3,5,4＇-tetrahydroxystilbene -2-O-β-D-glucoside） in Shengxuebao Granules by high-performance liquid chromatography （HPLC）. Methods HPLC was performed on an Agilent HC-C18 column （4.6mm×250mm×5μm） with a mobile phase of acetonitrile-water （18： 82） at a flow rate of 1.0 ml/min and a detection wavelength of 320 nm. Results The concentration of Polygorzurn Multiflorurn Glycoside showed a good linear relationship with peak area within 3. 580 - 114. 5 μg/ml （r=0. 999 94, n=6）, and the relative standard deviation （RSD） was 1.3 %; the average recovery rate was 100.3% （RSD= 1.76%）. Conclusion This HPLC method is simple and reliable and can be used for effective quality control of Shengxuebao Granules.
出处
《安徽中医学院学报》
CAS
2013年第5期86-88,共3页
Journal of Anhui Traditional Chinese Medical College
关键词
生血宝颗粒
高效液相色谱法
何首乌苷
Shengxuebao Granule
high-performance liquid chromatography
Polygonum Multiflorum glycoside
