摘要
目的用反相高效液相色谱法建立胃复春片中柚皮苷和新橙皮苷的含量测定方法。方法采用AgilentEclipseXDB．C18色谱柱（4．6mmX250mm，5tun），流动相为乙腈-水（20：80），流速为1．OmL·min-1，检测波长为283nm，柱温为40C。结果柚皮苷、新橙皮苷进样量分别在0．03234～0．71148μg和0．0196-O．43121xg范围内呈良好的线性关系（柚皮苷r=0．9999；新橙皮苷r=0．9996），平均回收率分别为98．07％和96．57％，RSD分别为0．62％和0．78％（n=6）。结论本方法快速，简便，结果准确可靠，重现性好，可用于胃复春片的质量控制。
OBJECTIVE To establish the analytical method for determining naringin and hesperidin in Weifu- ehun tablets by HPLC. METHODS The Agilent Eclipse XDB-C18 column （250mm x4. 6mm,51xm）was used and the mobile phase was acetonitile-water（20： 80） at a flow rate of 1.0mL ~ min-1. The detection wavelength was set at 283nm. The column temperature maintained at 40~C. RESULTS The standard curve showed linear in ranges of 0. 03234 ~ 0. 71148 Ixg and 0. 0196 ~ 0. 4312 Ixg for naringin and hesperidin, respectively （ naringin r = 0. 9999 and hesperidin r =0. 9996）. The average recoveries were 98.07% and 96. 57%. The RSD were 0. 62% and 0. 78% ,re- spectively （ n = 6）. CONCLUSION This method is quick, simple and aecurate with good reproducibility, which can be used for quality control of Weifuchun tablets.
出处
《海峡药学》
2013年第9期61-63,共3页
Strait Pharmaceutical Journal
