摘要
目的:探讨缬沙坦对门脉高压性胃病大鼠门静脉血管病变的作用.方法:48只SD大鼠随机分为假手术组、门脉高压性胃病模型组、缬沙坦预防组、缬沙坦正常剂量组和加倍剂量组.采用门静脉主干部分结扎法复制门脉高压性胃病模型.测量门静脉压力(portal venous pressure,PVP)、心率(heart rate,HR),免疫组织化学SABC法检测各组大鼠门静脉转化生长因子β1(transforming growth factor beta 1,TGF-β1)蛋白表达,Masson三色染色测定胶原纤维定量.结果:缬沙坦正常剂量组、加倍剂量组和预防组的PVP(9.54 cmH2O±0.80 cmH2O,9.04c m H2O±0.96 c m H2O,8.30 c m H2O±0.41cmH2O)明显下降,与造模组(13.22 cmH2O±0.96 cmH2O)比较差异具有统计学意义(P<0.01).假手术组门静脉TGF-1表达(5.73±0.87)和胶原纤维定量(0.82±0.54)最低,模型组TGF-β1表达(8.51±1.42)和胶原纤维定量(2.01±1.25)明显增高,缬沙坦正常剂量组、加倍剂量组和预防组TGF-β1表达(6.54±1.09,6.45±1.37,6.42±1.98)和胶原纤维定量(0.92±0.53,1.09±0.40,1.03±0.36)与造模组(8.51±1.42,2.01±1.25)比较差异均有统计学意义(P<0.05).结论:门脉高压性胃病时门静脉TGF-β1表达和胶原纤维定量升高,缬沙坦不仅可以降低门脉压力,而且抑制门静脉TGF-β1活性,降低胶原纤维合成,对门静脉血管病变具有一定治疗作用.
AIM： To investigate the effect of treatment with valsartan on portal vein lesions in rats with portal hypertensive gastropathy （PHG）. METHODS： Forty-eight SD rats were randomly divided into a sham operation group, a PHG model group, a valsartan prevention group, a normal dose valsartan group, and a double dose valsartan group. Partial portal vein ligation was used to induce PHG. Portal venous pressure （PVP） and heart rate （HR） were measured. Im- munohistochemistry was used to detect TGF-β1 protein expression in the portal vein, and Mas- son＇s trichrome technique was used to deter- mine the content of collagen fibers. RESULTS： The PVP decreased significantly in the normal dose valsartan group, double dose valsartan group and prevention group com- pared with the model group （13.32 cmH2O± 0.96 cmH20 vs 9.54 cm H2O± 0.80 cmH2O, 9.04 cmH2O ± 0.96 cmH2O, 8.30 cmH2O± 0.41 cmH2O, all P 〈 0.01）. TGF-β1 expression and collagen fiber con- tent were significantly higher in the model group than in the sham operation group （8.51 ± 1.42 vs 5.73 ± 0.87, 2.01 ± 1.25 vs 0.82 ± 0.54）; however, TGF-β1 expression and collagen fiber content were significantly lower in the valsartan normal dose group, double dose group and prevention group than in the model group （6.54 ±1.09, 6.45 ± 1.37, 6.42 ± 1.98 vs 8.51 ±1.42; 0.92 ±0.53, 1.09 ±0.40, 1.03± 0.36 vs 2.01± 1.25, all P 〈 0.05）. CONCLUSION： TGF-β1 expression and collagen fiber content in the portal vein increase in rats with PHG. Treatment with valsartan can not only reduce portal pressure and TGF-β1 expres- sion in the portal vein but also decrease collagen synthesis.
出处
《世界华人消化杂志》
CAS
北大核心
2013年第27期2765-2771,共7页
World Chinese Journal of Digestology
基金
上海市普陀区卫生系统自主创新科研基金资助项目
No.普科委[2010]33号~~
关键词
缬沙坦
门静脉高压性胃病
转化生长因子-Β1
胶原
Valsartan
Portal hypertensive gastrop-athy
Transforming growth factor-beta 1
Collagen
