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重组蛋白Lv-PEN-3的表达纯化与多克隆抗体制备

Expression,purification and polyclonal antibody preparation of recombinant Lv-PEN-3 protein
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摘要 以凡纳滨对虾的对虾素3(Lv-PEN-3)基因作为研究对象,分别进行了大肠杆菌重组表达载体的构建、重组蛋白的诱导表达与亲和纯化,以及多克隆抗体的制备等研究。研究结果显示:Lv-PEN-3可以被大肠杆菌正确表达,而且该重组蛋白具有良好的免疫刺激作用,可以刺激家兔产生相应的抗体。 In this paper, Litopenaeus vannamei penaeidin 3 was used as the research object. The construction of E. coli recombinant expression vectors, induced expression and purification of recombinant protein, and polyclonal anti- body preparation were investigated respectively. The results showed that Lv-PEN-] could be correctly expressed in E. coli expression system. And this recombinant protein possessed good immunity stimulation function. It could stimu- late rabbit to produce corresponding antibody.
作者 杜志强
机构地区 内蒙古科技大学数理与生物工程学院
出处 《浙江农业学报》 CSCD 北大核心 2013年第5期938-940,共3页 Acta Agriculturae Zhejiangensis
基金 2011年内蒙古自治区自然科学基金项目(2011BS0502)
关键词 凡纳对虾 对虾素 诱导表达 纯化 多克隆抗体制备 Litopenaeus vannamei penaeidin induced expression purification polyclonal antibody preparation
分类号 Q78 [生物学—分子生物学]
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参考文献9

  • 1Li CY,Song YL. Proline-rich domain of penaeidin moleculeexhibits autocrine feature by attracting penaeidin-positive gran-ulocytes toward the wound-induced inflammatory site [ J ].Fish and Shellfish Immunology^ 2010,29(6) : 1044-1052.
  • 2杜志强,王建英.凡纳对虾penaeidin 3抗菌肽基因的克隆及特性分析[J].广东农业科学,2012,39(8):152-153. 被引量：1
  • 3Du ZQ, Li XC, Wang ZH, et aL A single WAP domain(SWD) -containing protein with antipathogenic relevance inred swamp crayfish,Procambarus clarkii[ J]. Fish and Shell-fish Immunology, 2010,28(1) :134-142.
  • 4Sun C, Du XJ, Xu WT, et al. Molecular cloning and charac-terization of three crustins from the Chinese white shrimp, Fen-neropenaeus chinensis [ J ]. Fish and Shellfish Immunology,2010, 28(4) :517-524.
  • 5Li CY, Yan HY,Song YL. Tiger shrimp (Penaeus monodon)penaeidin possesses cytokine features to promote integrin-medi-ated granulocyte and semi-granulocyte adhesion [ J]. Fish andShellfish Immunology, 2010, 28(1) : 1-9.
  • 6Soderhall K. Prophenoloxidase activating system and melaniza-tion-a recognition mechanism of arthropods [ J]. Developmentaland Comparative Immunology, 1982, 6(4) :601-611.
  • 7Smith VJ. Phylogeny of whey acidic protein ( WAP) four-di-sulfide core proteins and their role in lower vertebrates and in-vertebrates [J ]. Biochemical Society Transactions,2011, 39(5):1403-1408.
  • 8Mu C, Zheng P, Zhao J, et al. A novel type III crustin (Cms.s2)identified from Chinese mitten crab Eriocheir sinensis [ J ].Fish and Shellfish Immunology t 2011,31(1) : 142-147.
  • 9Cerenius L,Lee BL, Soderhall K. The proPO-system; prosand cons for its role in invertebrate immunity [ J]. Trends inImmunology, 2008,29(6) :263-271.

二级参考文献6

  • 1Ren Q,Zhao X F,Wang J X.Identification of three different types of serine proteases (one SP and two SPHs) in Chinese white shrim[J]Fish Shellfish Immunol,2011,30(2):456-466.
  • 2Ren Q, Zhou J, Jia Y P,et al.Cloning and characterization of Rap GTPase from the Chinese white shrimp Fenneropenaeus chinensis [J].Dev Comp Immunol,2012,36(1):247-252.
  • 3Du Z Q,Ren Q,Zhao X F, et al.A double WAP domain (DWD)- containing protein with proteinase inhibitory activity in Chinese white shrimp, Fenneropenaeus chinensis [J].Comp Biochem Physiol B Biochem Mol Biol,2009, 154(2):203-210.
  • 4Du Z Q,Li X C,Wang Z H,et al.A single WAP domain (SWD)- containing protein with antipathogenic relevance in red swamp crayfish, Procambams clarkii [J]. Fish Shellfish Immunol,2010,28 (1):134-142.
  • 5Sun C,Du X J,Xu W T,et al.Molecular cloning and characterization of three crustins from the Chinese white shrimp, Fenneropenaeus chinensis[J].Fish Shellfish hmnunol,2010,28(4):517-524.
  • 6Jia Y P,Sun Y D,Wang Z H,et al.A single whey acidic protein domain (SWD)-containing peptide from fleshy prawn with antimicrobial and proteinase inhibitory activities [J].Aquaculture, 2008,284:246-259.
浙江农业学报
2013年 第5期

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