摘要
采用水培法研究2个油菜品种华油2790(铝敏感型)和中双7号(耐铝型),在4个铝浓度(0μmol/L、50μmol/L、100μmol/L、200μmol/L)和外源柠檬酸抑制剂Mersal(5μmol/L)复合处理下,油菜的抗氧化酶活性的变化。结果显示:在低浓度的单铝胁迫下,油菜体内的抗氧化酶类被激活,抗坏血酸过氧化物酶(APX)、多酚氧化酶(PPO)活性上升,但随着铝浓度的增加和处理时间的延长,均有所下降。加入Mersal后,与单铝处理相比,蛋白质含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)等酶的活性有所下降,而APX活性有所增加,过氧化物酶(POD)却没有明显变化。表明,在铝胁迫下,油菜的抗氧化酶系统被激活,使得蛋白质含量、SOD、CAT、APX等酶活性有所增加,以应对低浓度、短时间的铝胁迫。但随着铝浓度的增加和处理时间的延长,铝毒害加深。柠檬酸抑制剂Mersal加重了铝毒对油菜的伤害,破坏了油菜本身的抗氧化酶活性机制,且在铝敏感品种华油2790中表现更明显。
Under hydroponic conditions, activities of antioxidant enzymes were measured in the roots of two oilseed rape cuhivars Huayou 2790 （aluminum-sensitive） and Zhongshuang No. 7 （aluminum-tolerant） treated with different con- centrations （0 μmol/L, 50μmol/L, 100 μmol/L, 200 μmol/L） of aluminum alone or combined with citric acid inhibitor Mersal （5 μmol/L）. The results showed that the antioxidant enzymes activities of oilseed rape were activated under sole stress of aluminum at low concentration. The ascorbate peroxidase （APX） , polyphenol oxidase（PPO） activities were in- creased by short-term or low-concentration aluminum treatments. However, these enzymes activities gradually decreased with the extension of time or the increase of aluminum concentrations. Compared with sole aluminum treatment, combina- tion treatments with aluminum and Mersal significantly reduced protein content, activities of superoxide dismutase（SOD） and catalase（CAT）, and enhanced APX activity. The peroxidase（POD） activity did not change significantly. These data suggested that under aluminum stress, the antioxidant enzyme system was activated, leading to the increases of protein con- tent, the activities of SOD, CAT, APX to respond to low-concentration and short-term aluminum stress. But as aluminumconcentration rose and treatment time was extended, aluminum toxicity was aggravated. Inhibitor Mersal aggravated aluminum toxicity and diminished the role of antioxidant enzymes in alleviating aluminum toxicity in the oilseed rape cuhivars, especially in the sensitive cultivarHuayou 2790.
出处
《江苏农业学报》
CSCD
北大核心
2013年第5期957-966,共10页
Jiangsu Journal of Agricultural Sciences
基金
浙江省自然科学基金项目(Y307408)
关键词
油菜
铝胁迫
柠檬酸抑制剂
抗氧化酶活性
oilseed rape
aluminum stress
citric acid inhibitor
antioxidant enzyme activity
