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全烟气暴露与烟气冷凝物染毒方式的卷烟烟气体外微核测试结果比较 被引量：4

Comparison of Measurements from in vitro Micronucleus Assays for Cells Exposed to Whole or Condensate of Mainstream Cigarette Smoke
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摘要 采用VITROCELL系统建立了基于全烟气暴露的体外微核测试方法,并比较了全烟气暴露与烟气冷凝物染毒方式下卷烟主流烟气的体外微核测试结果。结果表明:该方法可以进行卷烟烟气的遗传毒性测试;在相同烟气剂量下,采用全烟气暴露方式测试的卷烟烟气遗传毒性大于烟气冷凝物染毒方式的测试结果;与传统的烟气冷凝物染毒方式相比,基于全烟气暴露方式的体外微核测试结果能够较全面地反映主流烟气的遗传毒性。 An in vitro micronucleus assay method for whole smoke exposure of mainstream cigarette smoke was established with VITROCELL system, and the measurements from in vitro micronucleus assays for cells exposed to whole or condensate of mainstream cigarette smoke were compared. The results indicated that the established method could be used for the genotoxicity testing of cigarette smoke. At the same smoke dosage, the genotoxicity of cigarette smoke determined with whole smoke exposure was greater than that with smoke condensate exposure. Comparing with traditional smoke condensate exposure, the measurements from in vitro micronucleus assay for whole smoke exposure relatively completely reflect the genotoxicity of mainstream cigarette smoke.
作者 李翔 尚平平 聂聪 谢复炜 刘惠民 谢剑平
机构地区 中国烟草总公司郑州烟草研究院
出处 《烟草科技》 EI CAS 北大核心 2013年第10期31-34,共4页 Tobacco Science & Technology
基金 国家自然科学基金资助项目"卷烟主流烟气和侧流烟气中颗粒物的尺度 化学组成与生物毒性研究"(21007094) 国家科技支撑计划课题"食品中热反应伴生危害物毒理学检测及风险评估关键技术研究"(2012BAK01B03) 国家烟草专卖局标准项目"卷烟全烟气暴露的体外毒理学测试方法"(2011B056)
关键词 卷烟主流烟气 全烟气暴露 烟气冷凝物 体外微核测试 遗传毒性 Mainstream cigarette smoke Whole smoke exposure Cigarette smoke condensate In vitromicronucleus assay Genotoxicity
分类号 TS416 [农业科学—烟草工业] R114 [医药卫生—卫生毒理学]
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参考文献22

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二级参考文献48

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共引文献28

同被引文献30

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烟草科技
2013年 第10期

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