摘要
[目的]探索汽油添加剂甲基叔丁基醚(methyl tert-butyl ether,MTBE)对加油站职业暴露人群DNA的损伤。[方法]选择华南地区8家加油站工作人员100名为调查对象(其中暴露组61人,对照组39人),进行健康检查和问卷调查;调查对象各抽取外周静脉血样5mL,分离血清和淋巴细胞,运用气相色谱-质谱(GC/MS)联用技术测定血清MTBE含量;运用彗星试验(comet assay),分析外周静脉血淋巴细胞DNA损伤情况;运用Pearson相关分析探索样本人群血清MTBE含量与淋巴细胞DNA损伤的相关性。[结果]接受体检的加油站调查对象未发现身体健康状况不合格者,均符合汽油从业人员的职业卫生要求;暴露组血清MTBE含量平均值为(6.230±2.369)μg/L,高于对照组[(5.164±2.139)μg/L](P<0.05);暴露组Olive尾矩平均值为(0.060±0.045)μm,也高于对照组[(0.039±0.038)μm](P<0.05);Pearson相关分析显示,暴露组血清MTBE含量与淋巴细胞DNA损伤程度之间呈正相关关系(r=0.859,P<0.05)。[结论]血清MTBE含量与淋巴细胞DNA损伤可能存在相关性。
[ Objective 3 To probe the DNA damages induced by a gasoline additive, methyl tert-butyl ether （MTBE）, in occupational exposed population. [ Methods ] Physical examination and questionnaire survey were conducted in 100 workers selected from 8 gas stations in South China. A 5 mL peripheral venous blood sample was collected from each worker. Serum and lymphocytes were separated. The content of serum MTBE was detected with gas chromatography-mass spectrometry （GC/MS）. Peripheral blood lymphocyte DNA damage was analyzed by comet assay. The relationship between serum MTBE content and lymphocyte DNA damage was explored using Pearson correlation analysis. [ Results ] All selected workers met the basic health requirements for gasoline station operators. The mean value of MTBE in the exposure group was （6.230 ± 2.369） μg/L, significantly higher than that in the control group, （5.164 ± 2.139） μg/L （P 〈 0.05）. The mean value of Olive tail moment in the exposure group was （0.060 ± 0.045）μm, significantly higher than that in the control group, （0.039 ± 0.038）μm （P 〈 0.05）. The results of Pearson correlation analysis showed a positive correlation between MTBE content and the degree of DNA damage （r=0.859, P〈 0.05）. [ Conclusion ] There is a correlation between serum MTBE content and lymphocyte DNA damage.
出处
《环境与职业医学》
CAS
北大核心
2013年第10期758-761,共4页
Journal of Environmental and Occupational Medicine
基金
国家环保部公益性行业科研专项基金项目(编号:201009008)
