摘要
建立了HPLC测定市售阿莫西林胶囊中有效成分的方法。采用Agilent C18柱(250mm×4.6mm,5Lm),以十八烷基键合硅胶为固定相,0.05mol·L-1磷酸二氢钾溶液(用2mol·L-1氢氧化钾溶液调节pH值至5.0)(A)-乙氰(B)(97.5∶2.5,V/V)为流动相。流速为1.0mL·min-1,柱温为25℃,检测波长为254nm,进样量为10LL。实验结果表明:回归方程为y=5.27x+2.83,r=0.9995(n=6),线性范围20—500Lg·mL-1,RSD为3.97%,样品中阿莫西林含量为821.3mg/g。此方法具有简单方便、灵敏度高、快速、准确性和重现性好等特点,可用于阿莫西林原料药及其制剂有效成分的质量控制。
The mothod of effective constituent in amoxicillin capsule in the markets was established by HPLC. The solution was separated by Agilent C18 column（250mm×4.6mm,5μm） with the stationary phase of oetadecyl bonded silica, potassium dihydrogen phosphate solution （0.05tool/L, in 2mol·L^-1 potassium hydroxide solution to adjust the pH to 5. 0）-aeetonitrile （97.5 ： 2.5, V/V） as mobile phase,flow rate of 1.0mol·min^-1, detection wavelength of 254nm, column temperature of 25C ,and injection volume was 10μL. The regression equation was y=5.27x 2.83,7-= 0. 9995 （n= 6）, and the linear range was from 20 to 500μg with RSD of 3. 97%. The montent of amoxicillin in sample was 821.3mg/g. The method is easy and conveninet, sensitive, fast and accurate with good reproducible, and the way can be used to quality control of the content of amoxicillin and its preparations.
出处
《光谱实验室》
CAS
2013年第6期2733-2736,共4页
Chinese Journal of Spectroscopy Laboratory
基金
国家自然科学基金资助项目(No.20771006)
安徽省高校省级科学研究项目(项目编号:KJ2013B164
KJ2010B126)
