摘要
目的:检测促红细胞生成素(Erythropoietin,EPO)对荷黑色素瘤小鼠免疫功能的影响。方法:将C57BL/6小鼠随机分为3组:正常对照组、盐水处理组和EPO处理组,盐水处理组和EPO处理组小鼠皮下接种黑色素瘤细胞B16。17天后,检测各组小鼠外周血中红细胞(RBC)、血红蛋白(Hb)、红细胞压积(Hct)和白细胞(WBC)数量及脾脏脏器指数;应用流式细胞术检测脾细胞CD4+、CD8+T细胞亚群的分布;ELISA检测血清中IL-2和TNF-α细胞因子的水平。结果:盐水处理组荷瘤小鼠外周血中RBC、Hb和Hct含量明显低于正常对照组小鼠(P<0.05),应用EPO可明显提高荷瘤小鼠外周血中RBC、Hb和Hct含量及脾脏脏器指数(P<0.05),三组小鼠外周血中WBC数量无明显差别(P>0.05);盐水处理组及EPO处理组荷瘤小鼠脾脏CD4+T细胞百分率及CD4+/CD8+T细胞比值明显低于正常小鼠(P<0.05)。EPO处理组与盐水处理组相比,CD4+T细胞百分率无差别(P>0.05),而CD8+T细胞百分率下降(P<0.05),致使EPO处理组小鼠CD4+/CD8+T细胞比值升高(P<0.05);盐水处理组及EPO处理组荷瘤小鼠血清中TNF-α、IL-2含量明显低于正常小鼠(P<0.05),EPO处理组小鼠血清中IL-2水平与盐水处理组相比增高(P<0.05),但该两组小鼠血清中TNF-α含量无明显差别(P>0.05)。结论:EPO在改善荷瘤小鼠贫血状态的同时,还可通过提高外周血CD4+/CD8+T细胞比值和IL-2含量而增强小鼠的免疫功能。
Objective：To study the effects of erythropoietin （EPO） on immune function in a murine melanoma model. Meth- ods：C57BL/6 mice were randomly divided into 3 groups： normal control group, saline-tre/tted group and EPO-treated group. Mice of saline-treated group and EPO-treated group were inoculated with melanoma cell B16. The changes of red blood cell（ RBC）, hemoglobin （Hb）, hematocrit （Hct） and white blood cell（WBC） in peripheral blood, spleen index were observed. The subpopulations of CD4 ＋ and CD8 ＋ T-cell in mice spleen was performed by flow cytomery. Serum concentrations of interleukin （IL） -2 and tumor necrosis factor （TNF）-α of 3 groups was measured by ELISA. Results：A significantly greater reduction in the levels of RBC, Hb and Hct in periph- eral blood was observed in saline-treated mice compared to the normal control mice （ P 〈 0, 05 ） . EPO treatment increased the levels of RBC, Hb and Hct in peripheral blood and spleen index in tumor-bearing mice （ P 〈 0. 05 ）. The levels of WBC in peripheral blood was not different in 3 groups（ P 〉 0.05 ） ; CD4＋T-cell percentage and CD4 ＋/CD8 ＋ ratio of spleen cell was significantly decreased in tumor-bearing mice compared to the normal control group（ P 〈 0.05 ）. CD4＋ T-cell percentage of mice spleen was not different （P 〉 0. 05 ） , but CD8 ＋ T-cell percentage was significantly decreased leading to increase of the CD4 ＋/CD8 ＋ ratio in EPO-treated group com- pared to the saline-treated group（ P 〈 0.05 ） ; the serum concentrations of IL-2 and TNF-α was significantly decreased in tumor-bearing group compared to the normal control group （ P 〈 0.05 ）. The concentration of IL-2 was significantly increased in EPO-treated group compared to the saline-treated group （ P 〈 0. 05 ）, while that of TNF-α was not different in both study groups （ P 〉 0. 05 ）. Conclu- sion：These results suggest that EPO treatment can correct the anemia, and also improve immune function in tumor-bearing mice by in- creasing the CD4 ＋/CD8 ＋ ratio and IL-2 concentrations of peripheral blood.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2013年第1期35-38,共4页
Chinese Journal of Immunology
基金
河北省卫生厅重点科技研究计划项目(No.20120289)
