摘要
本试验采用 F- BSG法制备染色体标本 ,对七份苎麻野生材料进行染色体核型及 Giemsa C-带带型研究。结果表明 :七份材料中 1 - 1、1 - 2的染色体数目众多 ,分别为 5 6和 42 ,其余 5份材料为 2 8,均为近端着丝点型 ,核型公式 1 - 1为 2 n=5 6 =5 6 st,1 - 2为 2 n=42 =42 st,其余 5份材料均为 2 n=2 8=2 8st。臂指数均大于 75 % ,N.F.值与染色体条数相等 ,1 - 1 ,1 - 2 ,1 - 3的染色体长度比大于 2 ,核型属 4B类型 ,2 -1 ,2 - 2 ,2 - 3,2 - 4的染色体长度比小于 2 ,核型属于 4A类型。 Giemsa C-带带型单一 ,长臂均为着丝点带 ,且染色区段较长 ,短臂均为全带。染色体相对长度组成 1 - 1、1- 2、1 - 3的分别为 2 n=5 6 =8L+ 1 6 M2 + 2 2 M1 + 1 0 S、2 n=42 =2 L+ 2 2 M2 + 1 4M1 +4S、2 n=2 8=2 L+ 1 4M2 + 6 M1 + 6 S,其余 4份材料均为 2 n=2 8=2 L+ 1 2 M2 + 1 2 M1 +2 S。本文还对苎麻染色体的基数进行了探讨。
The Karyotype and Giemsa C-banding pattern of seven wild resources of ramie(Boehmeria nivea L.)are studied by flame drying-BSG banding.The results showed that chromosome number of 1-1,1-2 and others materials are 56、42 and 28,respctively.All pattern of Giemsa C-banding are short top dot-band.The karyotype formula of 1-1,1-2and others materials are 2n=56 56st,2n=42=42st and 2n=28=28st,respectively.The arm index of all materials is larger than 75%, While values of N、F are equal to chromosome numbers. The length ratios of chromosome of 1-1, 1-2 and 1-3 are bigger than 2,Which belong to “4B”types in the karyotype of chromosome,The length ratios of chromosome of 2-1,2-2,2-3 and 2-4 are less than 2,Which belong to“4A”types in the karyotype of chromosome.The pattrn of Giemsa C-banding is singleness,all of longer arms are centrometric dot-band,While all of shorter arms are full band.The composition of relative length of chromosome of 1-1,1-2,1-3 and other materials are 2n=56=8L+16M 2+22M 1+10S,2n=42=2L+22M 2+14M 1+4S,2n=28=2L+14M 2+6M 1+6S,and 2n=28=2L+12M 2+12M 1+2S.The basic number of chromosome of Boehmeria nivea L. is studied.
出处
《中国麻作》
北大核心
2000年第2期6-11,共6页
China's Fiber Crops
