摘要
目的：右旋糖酐酶催化水解工艺替代右旋糖酐发酵工艺中的盐酸水解.方法：以药用右旋糖酐70（1）、右旋糖酐40（2）和右旋糖酐20（3）的含量为指标,采用HPLC测定指标成分含量,酶催化温度、pH、底物浓度、酶浓度、反应时间为考查因素,确定药用级右旋糖酐水解的酶催化工艺.条件与结果：制备右旋糖酐70（1）、右旋糖酐40（2）和右旋糖酐20（3）的酶催化工艺：底物浓度为7％,酶浓度为2U/mL,于pH 5.0、200r/min、35℃条件下分别反应60～70min、70～80min、90～110min.结论：右旋糖酐酶法催化工艺简单可控,操作性强,适合大规模生产.
Objective： Dextranase catalyzed hydrolysis process replace hydrochloric acid hydrolytic section of dextran fermentation process. Method： With the content of the pharmaceutically dextran 70 （1）, dextran 40 （2）, dextran 20 （3） as an index, which was determined by HPLC, enzymatic hydrolysis process of pharmaceutical dextran was determined by catalysis temperature, pH, substrate concentration, enzyme concentration, reaction time as factors. Condition and Result： Enzymatic process to prepare dextran 70 （1）, dextran 40 （2） and dextran 20 （3）： the substrate concentration of dextran was 7%, and enzyme concentration was respectively 2U/mL. Under the conditions of pH 5.0, 200r/min, 35 ℃, reaction time was 60-70min, 70-80min,90-110rain, respectively. Conclusion： Enzymatic process was simple, controllable, operable and suitable for mass production.
出处
《安徽化工》
CAS
2013年第5期17-21,共5页
Anhui Chemical Industry
基金
安徽省长三角联合科技攻关项目(10140702001)
国家级大学生创业训练项目(201210359077)
