摘要
目的以自制载量子点(QDs)乳酸/羟基乙酸共聚物(PLGA)微泡(MBQDs/PLGA)为光敏剂,超声(US)联合光动力疗法(PDT)处理人卵巢癌SKOV3细胞,研究细胞凋亡机制。材料与方法以双乳化法制备MBQDs/PLGA,采用MTT法比较QDs与MBQDs/PLGA的细胞毒活性,确定处理条件。在选择条件下处理SKOV3,行HE染色观察细胞受损情况,以流式细胞仪双染检测细胞凋亡情况。结果以PDT能量密度为180.0 J/cm2、US声强为0.5 W/cm2(1.0 MHz,10 s)处理细胞,US-PDTMBQDs/PLGA和PDT-MBQDs/PLGA两组显微镜下可见细胞变形,细胞间失去彼此连接;透射电镜下可见致密的染色质沿核膜下聚集,有凋亡小体形成;最大细胞凋亡率为(22.17±0.38)%。结论 MBQDs/PLGA介导的PDT对SKOV3细胞具有增殖抑制作用,且低功率超声辐照因声孔效应能协同增效MBQDs/PLGA的PDT作用。
Purpose To explore the apoptosis mechanism of human ovarian cancer cells SKOV3 processed with ultrasound （US） combined with photodynamic therapy （PDT）, with self-contained lactic acid/glycolic acid （PLGA） microbubbles （MBQDs/PLGA） containing quantum dots （QDs） as a photosensitizer. Materials and Methods MBQDs/PLGA was prepared with double emulsion method, and MTT was used to compare the cytotoxic activity difference between QDs and MBQDs/PLGA, to determine the proper treatment condition. SKOV3 cells were treated under selective conditions, cell damage manifestation was observed with HE staining, and double staining flow cytometry was used to detect cell apoptosis. Results When cells were treated with PDT with energy density of 180.0 J/cm^2 and US with sound intensity of 0.5 W/cm^2（1.0 MHz, 10 s）, cell deformation could be observed under a microscope in both US-PDT-MBQDs/PLGA group and PDT-MBQDs/PLGA group, and cell connections were absent between cells; transmission electron microscope showed dense chromatin gathered along the nuclear membrane, with the formation of apoptotic bodies also be displayed. Maximum apoptosis rate was （22.17±0.38）% Conclusion MBQDs/PLGA-mediated PDT contains proliferation inhibition effect for SKOV3 cells, which can be synergied with low-power ultrasonic irradiation due to sonoporation effect.
出处
《中国医学影像学杂志》
CSCD
北大核心
2013年第10期729-732,736,共5页
Chinese Journal of Medical Imaging
关键词
卵巢肿瘤
肿瘤细胞
培养的
超声疗法
量子点
微气泡
光化学疗法
细胞增殖
细胞凋亡
体外研究
Ovarian neoplasms
Tumor cells, cultured
Ultrasonic therapy
Quantum dots
Microbubbles
Photochemotherapy
Cell proliferation
Apoptosis
In vitro
