摘要
利用新鲜材料的徒手切片直接观察、5% Na OH水溶液显色、荧光显微镜观察和戊二醛及锇酸固定薄切片法对大黄根茎蒽醌类化合物进行了组织化学定位研究。结果发现大黄根茎内蒽醌类化合物主要积累在次生木质部的木射线和次生韧皮部的韧皮射线细胞中。根据显色程度的不同 ,可以判断早期形成的维管射线细胞蒽醌类化合物的含量较晚期形成的射线细胞的含量高 。
Localization of anthraquinones was investigated in rhizome of Rheum officinale with 5% NaOH solution coloring,fluorescence microscopy,and semi thin section method were fixed in glutaraldehyde and OsO 4.Anthraquinones were located in the vascular ray of secondary phloem and secondary xylem.Mature ray cells showed deeper color than immature ray cells,it suggested that anthraquinones content were higher in mature ray cells than in immature ray cells,and higher in biennial rhizome than in annual rhizome.
出处
《西北植物学报》
CAS
CSCD
2000年第6期1082-1085,T007,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
陕西省教委专项基金资助项目
关键词
大黄
根茎
组织化学
蒽醌类化合物
Rheum officinale Bail
rhizome
histochemistry
anthraquinones
