摘要
光周期信号在昆虫的环境适应中发挥着重要作用,昆虫能够通过感受光周期的变化来调节体内生理生化过程,以适应环境的变化。为明确光周期对中华通草蛉Chrysoperla sinica越冬成虫滞育解除过程中酶活力的影响,本研究测定了长光周期(15L:9D)和短光周期(9L:15D)条件下,成虫体内过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、Na^+K^+-ATP酶和乳酸脱氢酶(LDH)4种重要酶活力的变化。结果表明:中华通草蛉雌、雄成虫CAT活性在长光周期处理5d达最高值后呈下降趋势;短光周期处理CAT活性在处理5d达最高值,且高于长光周期处理,在处理10 d迅速下降至最低值,且均显著低于长光周期处理的CAT活性(P=0.005),后迅速上升并在处理15 d(P<0.05)和20 d(P<0.005)活性显著高于长光周期处理。雌、雄成虫SOD活性在长光周期下处理10d达最高值,且显著高于对照(P<0.001),且除处理5 d雄虫SOD活性与对照无显著性差异外(P=0.558)其余处理活性均显著低于对照(P<0.05)。雌成虫长光周期处理5 d的SOD活性显著低于短光周期(P<0.001),其余处理活性均显著高于短光周期;雄成虫长光周期下处理的SOD活性均高于短光周期下,且处理5 d(P=0.04),15 d(P<0.001)和20d(P=0.003)的活性差异显著。两种光周期条件下雌、雄成虫Na^+K^+-ATP酶活性随处理时间延长呈上升上下降-上升趋势,且均显著高于处理0 d成虫酶活性(P<0.001);短光周期处理不同时间Na^+K^+-ATP酶活性均高于长光周期处理,且除雄成虫处理15 d无显著性差异(P=0.142)外,其余均差异显著(P<0.05)。两种光周期条件下雌、雄成虫LDH活性随处理时间延长呈下降趋势,且均显著低于对照(P<0.001)。中华通草蛉越冬成虫在长、短两种光周期条件下体内酶活力的差异可能是影响两种光周期下成虫滞育解除过程中体内不同生化物质含量与生殖状态的重要因子。
Photoperiodic cues play important roles in insect environmental adaption, and insects can regulate their physiological processes to adapt environmental changes through perceiving the changes of photoperiod. In order to investigate the mechanisms of physiological adaptation to photoperiod changes, we detected the activities of catalase （CAT）, superoxide dismutase （SOD）, Na＋K＋-ATPase and lactic dehydrogenase （LDH） of overwintering adults of Chrysoperla sinica （Tjeder） in the progress of diapause termination under the long （15L∶9D） and short photoperiods （9L∶15D） in the laboratory conditions. The CAT activity of both female and male adults reached a peak at 5 d after treatment and was higher under the long photoperiod than under the short photoperiod, with significant difference at 15 d （P〈0.05） and 20 d after treatment （P〈0.005）. However, the CAT activity was significantly lower under the long photoperiod than under the short photoperiod at 10 d after treatment （P=0.005）. The SOD activity of female adults was significantly lower under the long photoperiod than under the short photoperiod at 5 d after treatment （P〈0.001）. The SOD activity of male adults under the long photoperiod was significantly higher at 5 d （P=0.04）, 15 d （P〈0.001） and 20 d after treatment （P=0.003） than that under the short photoperiod. The LDH activity decreased with the time under the two photoperiods, and the activities in both treatments were significantly lower than that in adults treated for 0 d （P〈0.001）. The results indicated that the differences of enzyme activities of Ch. sinica under the long and short photoperiods may be the main physiological factor triggering the content of bio-chemical substances in the adult body and reproductive status during the adult diapause termination.
出处
《昆虫学报》
CAS
CSCD
北大核心
2013年第9期982-988,共7页
Acta Entomologica Sinica
基金
国家重点基础研究发展规划(“973”计划)项目(2013CB127605)
国家公益性行业(农业)科研专项(201303024)
山东省小麦产业技术体系项目
