摘要
目的探讨在生理状况下不同剂量活性维生素D3(VD3)干预下老年大鼠是否发生细胞自噬,以及发生自噬的情况。方法 18月龄SD大鼠32只按体质量随机分为4组:A组:对照组;B组:低剂量活性VD3组[(0.01μg/kg)/d];C组:中剂量活性VD3组[(0.1μg/kg)/d];D组:高剂量活性VD3组[(0.4μg/kg)/d]。经过5个月灌胃不同剂量活性VD3干预后处死,取1mm3肝脏组织4℃保存,取其余肝组织,生理盐水冲洗,锡纸包裹-80℃保存。透射电镜观察大鼠肝脏细胞自噬情况。通过western-blot的方法测定自噬小体标志物LC3对细胞的自噬活性进行半定量分析。结果在生理状况下不同剂量活性维生素D的干预导致大鼠细胞自噬泡与胞浆的比值:A、B、C、D四组大鼠自噬泡与胞浆比值之间差异有统计学意义(P<0.05)。两两比较结果,A组与B、C、D组差异都有统计学意义(P<0.05),B组和C组之间差异没有统计学意义(P>0.05),B组和C组都与D组差异有统计学意义(P<0.05)。LC3II/LC3I结果与大鼠细胞自噬泡与胞浆的比值相同。结论在生理状况下长期大剂量给予不同剂量活性维生素D可对老年大鼠细胞自噬产生不同影响。
Objective To explore whether under the physiological condition given different dosage of 1-25 （OH） 2VD3 in aged rats could lead different autophagy. Method Thirty-two male 18-month-old SD rats were randomly divided into 4 groups： A： control group, B： Low dosage of i-25 （OH） 2VDa, [0.01μg/kgxd]; C： middle dosage of 1-25 （OH） 2VD3 [0.1μg/kg · d]; D： high dosage of 1-25 （OH） 2VD3 [0.4, μg/kg · d]. Given the rats with different dosages of 1-25 （OH） 2VD3. Take the samples after five months. Autophagsomes Were observed with Transmission Electron Microscopy, Western-blot detect the expression of LC3 which is the marker proteins for autophagy. And then comparing different of rats LC3 expression occurrence autophagy and degree of difference in different groups. Result Under physiological conditions given different dosage of 1-25 （OH） 2VD3 in four groups lead the proportions of autophagosome to the total area of cytoplasm have significant difference （P〈0：05） .Pairwise comparison results The proportions of autophagosome to the total area of cytoplasm in group A was significantly lower than any other groups （P〈0.05） .There have no statistical different between group B and group C （P〉0.05） .There have significant difference between group B and group D,group C and group D （P〈0.05） .The result of LC3II/LC3I were as same as the result of proportions of autophagosome to the total area. Conclusion To old rats under physiological condition long-term given different large dosage of VD3 can lead different autophagy degree in different groups.
出处
《中国医药指南》
2013年第27期1-4,共4页
Guide of China Medicine
基金
山西省自然科学基金(项目编号:2010011054-2)
