摘要
目的探究Foxo1在糖尿病大鼠肾脏的表达和脂质代谢的关系。方法构建1型糖尿病大鼠模型,喂养2个月后处死,取肾脏组织,免疫组织化学和Western blot检测Foxo1、Akt和SREBP-1在肾脏的表达;油红O染色及组织脂质定量检测肾组织中脂质含量。结果糖尿病大鼠出现明显的多饮、多食、多尿症状,体重较正常组明显下降,血糖、血甘油三酯及血尿素氮升高。免疫组织化学检测显示,磷酸化Foxo1和Foxo1表达定位于肾小管上皮细胞。Western blot检测可见与正常组大鼠相比,磷酸化Foxo1在糖尿病大鼠表达升高;而总Foxo1在两组间表达未见差异。相似地,磷酸化Akt和SREBP-1在糖尿病组大鼠表达也均明显升高。油红O染色表明脂滴沉积于糖尿病大鼠肾小管上皮细胞,定量甘油三酯测定显示糖尿病组肾脏脂质含量高于正常对照大鼠。结论磷酸化Foxo1在糖尿病大鼠肾小管上皮细胞表达升高,可能和SREBP-1上调及细胞内脂质沉积相关。
Aim To investigate the expression of Foxol and its function on lipid metabolism in the kidney of diabetic rats. Methods Diabetic rats models were constructed with STZ and fed for 2 months. After sacrificed, the expression of Foxol, Akt and SREBP-1 in the kidney was detected by the methods of immunohistoehemistry and Western blot, respectively. Again, Oil Red O staining and triglyceride quantitative kit were used to determine the lipid content in renal tissue. Results Diabetic rats presented such characteristics of diabetes as polydipsia, polyphagia, polyuria and weight loss. The blood specimens＇ measurement showed increased glucose, triglyeeride and BUN. As the result of immunohistochemistry, phospho-Foxol and total Foxol were located in the renal tubular cells.Western blot quantitatively revealed the phosphorylation of Foxol of renal tubular cells was enhanced in diabetic rats in comparison with normal rats. Similarly, both phospho-Akt and SREBP-1 were significantly upregulated in diabetic rats. Then Oil Red O staining showed the site of lipid accumulation was located in renal tubular cells. Compared with normal rats, increased renal triglyceride was proved in diabetic rats using triglyceride detection kit. Conclusion Phospho- rylation of Foxol was increased in renal tubular cells of diabetic rats, which might be related to the up-regulation of SREBP-1 and lipid deposit.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2013年第11期1519-1523,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学家基金资助项目(No 81100517)
河北省自然科学基金资助项目(No H2012206008)
