摘要
目的:建立大黄酸水解提取物的HPLC指纹图谱分析方法,为其质量评价提供科学依据。方法:采用Agilent Eclipse XDB-C18ODS色谱柱(150mm×4.6mm,5μm)分离,甲醇-0.6%冰醋酸水溶液梯度洗脱;流速:0.8mL/min;检测波长:254nm。结果:7种不同产地样品的指纹图谱通过相似度计算,相似度均在80%以上。结论:该方法简便、灵敏、准确,可为该药的质量控制提供依据。
Objective:To establish fingerprint chromatograms of acid hydrolyzed extracts of Rheum palmatum by HPLC.Method:Rheum palmatum was hydrolyzed by acid.Agilent Eclipse XDB-C18 ODS column(150mm×4.6mm,5μm)was used,and mobile phase was methanol-0.6% acetic acid aqueous gradient elution with a flow rate of 0.8mL/min.The detective wavelength was 254nm.Result:The similarities of seven acid hydrolyzed extracts of Rheum palmatum from different origins were above 80%.Conclusion:The method is simple,sensitive and accurate and it can be used for the quantification control of acid hydrolyzed extracts of Rheum palmatum.
出处
《中医药信息》
2013年第5期71-73,共3页
Information on Traditional Chinese Medicine
基金
哈尔滨医科大学大学生创新课题(2012084)