摘要
目的:探讨致炎剂联合降钙素基因相关肽对大鼠硬脑膜神经源性炎症的影响,寻求最佳慢性偏头痛的实验模型。方法:24只SD雄性大鼠,随机分为生理盐水组(NS)、降钙素基因相关肽组1(CGRP 1,10-3M)、降钙素基因相关肽组2(CGRP 2,10-4M),以及IS(20μL)+CGRP(10μL,10-4M)组,每组各6只,通过甲苯胺蓝染色法,观察肥大细胞脱颗粒,采用伊文氏蓝荧光显像法,观察大鼠硬脑膜血管渗出,采用多普勒激光血流仪检测法,观察各组硬脑膜动脉血流量变化。结果:与NS组相比,CGRP1、CGRP2、IS+CGRP组肥大细胞脱颗粒数和比率、脑血流量均明显升高,P<0.05,并且硬脑膜荧光红斑明显增多;与CGRP1、CGRP2组相比,IS+CGRP组肥大细胞脱颗粒数和比率、脑血流量均明显升高,P<0.05,并且硬脑膜荧光红斑明显增多。CGRP两组相比,上述指标比较,差异没有统计学意义,P>0.05。结论:IS+CGRP能够明显刺激大鼠硬脑膜发生神经源性炎症反应,可以作为慢性偏头痛动物实验模型。
Objective： To study the effect of inflammatory agents combined with calcitonin gene related peptide on the dural neu- rogenic inflammation in rats. Methods： The 24 male rats were randomized into the normal saline group （NS）, the calcitonin gene related peptide group 1 （CGRP1, 10-3M）, the calcitonin gene related peptide group 2 （CGRP2, 10-4M） and the IS＋CGRP group, 6 rats per group. The detection of mast cell degranulation by toluidine blue staining, the dural vascular leakage by Evans blue fluorescence imaging, the dural artery blood flow by laser Doppler flowmetry. Result： compared with the NS group, the mast cell degranulation number and ratio, the cerebral blood flow were significantly increased with statistical difference, P〈0.05, and the dural fluorescence erythema was obviously increased in the CGRP1, CGRP2, IS＋CGRP groups; compared with the CGRP 1 and CGRP 2 group, the mast cell degranulation number and ratio, the cerebral blood flow were significantly increased with statistical difference, P〈0.05, and the dural fluorescence erythema was obviously increased in the IS＋CGRP groups. The difference between the two groups of CGRP about the above parameters were no statis- tical significance, P〉0.05. Conclusion： IS＋CGRP can significantly stimulate rat dural neurogenic inflammation reactions and can regard it as the animal model for chronic migraine.
出处
《现代生物医学进展》
CAS
2013年第26期5046-5049,共4页
Progress in Modern Biomedicine
