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特应性皮炎模型小鼠的生长繁殖及血液生理生化指标 被引量:1

Growth, reproduction and blood physiological and biochemical indexes in an atopic dermatitis mouse model
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摘要 背景:Nc/Nga小鼠皮损、病理和免疫学特征等均与人类特应性皮炎相一致,作为特应性皮炎动物模型其研究价值日益显现。目前国内尚无Nc/Nga小鼠的生理生化指标等相关数据报道。目的:观察特应性皮炎动物模型Nc/Nga小鼠的生长繁殖性能以及血液生理、生化指标。方法:统计Nc/Nga小鼠1-3胎的繁殖指标数据,包括每胎产仔数、离乳率、怀孕率及胎间隔。分别称取60只1-56 d Nc/Nga仔鼠的体质量,雌雄各半,绘制其生长表格。Nc/Nga鼠眼眶采血,测定其血常规和生化值。结果与结论:Nc/Nga小鼠平均交配分娩间隔为(25.8±3.1)d,平均产仔数为(7.5±2.5)只,平均离乳率为(97.2±1.2)%、平均怀孕率为(97.0±1.4)%,1-3胎间差异无显著性意义(P>0.05)。Nc/Nga小鼠的体质量随着日龄的增长而逐渐增加,断奶后一两周内增重最为迅速,6周后体质量雌雄间差异无显著性意义(P>0.05)。同一日龄的雌雄间血常规和生化值比较,雌性小鼠红细胞、血红蛋白明显高于雄性小鼠;雄性小鼠血小板计数明显高于雌性小鼠(P<0.05);雌性小鼠三酰甘油、白蛋白水平明显高于雄性小鼠(P<0.05)。提示性别和年龄都会影响Nc/Nga小鼠的血液生理和生化值指标。 BACKGROUND: The skin lesions, Pathological and immunological characteristics of Nc/Nga mice are consistent with human atopic dermatitis, and as an atopic dermatitis animal model, it has great research value. But there are no reports on the physiological and biochemical parameters of Nc/Nga mice in China. OBJECTIVE: To observe the growth and reproduction and blood physiological and biochemical parameters of atopic dermatitis model of NC/Nga mice METHODS: The data of reproductive performance of NC/Nga mice from the first to the third generation was analyzed, including mean litter size, weaning rate, pregnancy rate and generation interval. The body mass of 60 Nc/Nga mice with 1 56 days old was measured, 30 mice of female and male, then the growth curve was draw. The blood samples from the infraorbital vessels were collected to detect the blood physiological and biochemical parameters in the mice. RESULTS AND CONCLUSION: The mean delivery interval of Nc/Nga mice was (25.8±3.1) days, mean litter size was (7.5±2.5) mice, mean weaning rate was (97.2±1.2)%, the mean pregnancy rate was (97.0±1.4)%, and there was no significant difference among the three generations of these mice (P 〈0.05). The body mass of Nc/Ngamice Was increased with the time increasing of days, the body mass of the mice was maximal within 1-2 weeks post-weaning, and there was no significant difference in body mass between males and females at 6 weeks post-weaning (P〉0.05) Comparison of the blood physiological and biochemical parameters of mice at the same age between males and females showed that the levels of red blood cells and hemoglobin in female mice were significantly higher than those in the male mice; the p latelet count in the male mica was significantly higher than that in the female mica (P 〈 0.05) the triglycerides and albumin levels in the female mice were higher than those in the male mice (P 〈 0.05). The results indicate that the gender and age may influence the blood physiological and biochemical parameters of Nc/Nga mica.
出处 《中国组织工程研究》 CAS CSCD 2013年第41期7284-7289,共6页 Chinese Journal of Tissue Engineering Research
基金 湖南省科研条件创新专项计划资助项目(2011TT2019)~~
关键词 组织构建 组织构建实验造模 Nc Nga鼠 特应性皮炎 生长率 血液生理生化指标 分娩间隔 产仔数 离乳率 省级基金 skin diseases models animal growth breading blood chemical analysis
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参考文献41

  • 1云晴,杨高云,乌日娜.小鼠模型在特应性皮炎方面的研究进展[J].临床和实验医学杂志,2012,11(22):1827-1829. 被引量:4
  • 2付萌,赵小东,廖文俊,刘玉峰.特应性皮炎的治疗现状与进展[J].临床皮肤科杂志,2006,35(6):410-412. 被引量:12
  • 3Shiga T,Yokogawa M,Nakajima K. Topical tacrolimus treatment does not facilitate photocarcinogenesis in cancer-prone mice[J].{H}Journal of Dermatological Science,2012,(02):112-115.
  • 4Matsuoka A,Kato T,Soma Y. Analysis of T cellreceptor (TCR) BV-gene clonotypes in NC/Nga mice developing dermatitis resembling human atopic dermatitis[J].{H}Journal of Dermatological Science,2005,(01):17-24.
  • 5Jiang J,Yamaguchi T,Funakushi N. Oral administration of Yokukansan inhibits the development of atopic dermatitis-like lesions in isolated NC/Nga mice[J].{H}Journal of Dermatological Science,2009,(01):37-42.
  • 6尤立平,刘永生.特应性皮炎动物模型NC/Nga鼠的发现与研究进展[J].国外医学(皮肤性病学分册),2003,29(1):22-24. 被引量:7
  • 7Yamashita H,Makinoa T,Mizukami H. Pharmacological characterization of a chronic pruritus model induced by multiple application of 2,4,6-trinitrochlorobenzene in NC mice[J].{H}EUROPEAN JOURNAL OF PHARMACOLOGY,2007,(1-3):233-239.
  • 8Gao XK,Nakamura N,Fuseda K. Establishment of al ergic dermatitis in NC/Nga mice as a model for severe atopic dermatitis[J].J Biol Pharm Bul,2004,(09):1376-1381.
  • 9Matsuoka H,Maki N,Yoshida S. A mouse model of the atopic eczema/dermatitis syndrome by repeated application of a crude extract of house-dust mite Dermatophagoides farinae[J].J Al ergy,2003,(02):139-145.
  • 10王俊风,施美莲,赵立虎,孙宗国,符杰,高静华,郭建光,徐平.无菌级C3H/OrlSlac小鼠生长繁殖、主要脏器参数以及血液生理生化指标的测定分析[J].中国实验动物学报,2011,19(2):124-128. 被引量:7

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  • 1Flohr C, Mann J. New insights into the epi- demiology of childhood atopic dermatitis [J]. ALLERGY, 2014, 69(1SI) :3 - 16.
  • 2Xu F, Yan S, Li F, et al. Prevalence of childhood atopic dermatitis: an urban and ru- ral community-based study in Shanghai, Chi- na[J]. PLoS One, 2012, 7(5) :e36174.
  • 3Choi JK, Kim S. Rutin suppresses atopic dermatitis and allergic contact dermatitis [ J 1. Experimental Biology and Medicine, 2013, 238(4) :410 -417.
  • 4Matsuda H, Watanabe N, Geba GP, et al. Development of atopic dermatitis-like skin le- sion with IgE hyperproduction in NC/Nga mlce[J]. Int Immunol, 1997, 9(3): 461 -466.
  • 5Choi JH, Park BH, Kim HG, et al. Inhibi- tory effect of Psidium guajava water extract in the development of 2, 4-dinitrochloro- benzene-induced atopic dermatitis in NC/ Nga mice[J]. Food Chem Toxicol, 2012, 50 ( 8 ) : 2923 - 2929.
  • 6Pokharel YR, Lim SC, Kim SC, et al. Sopungyangjae-tang inhibits development of dermatitis in nc/nga mice [ J ]. Evid Based Complement Alternat Med, 2008, 5 (2) : 173 - 180.
  • 7Ishii N, Shirato M, Wakita H, et al. Anti- pruritic effect of the topical phosphodiester- ase 4 inhibitor E6005 ameliorates skin le- sions in a mouse atopie dermatitis model [J]. J Pharmacol Exp Ther, 2013, 346 (1): 105-112.
  • 8Matsumoto K, Mizukoshi K, Oyobikawa M, et al. Establishment of an atopic der- matitis-like skin model in a hairless mouse by repeated elicitation of contact hypersensitivity that enables to conduct functional analyses of the stratum corneum with various non-invasivc biophysical in- struments[ J]. Skin Res Technol, 2004, 10(2) : 122 - 129.
  • 9Man MQ, Hatano Y, Lee SH, et al. Characterization of a hapten-induced, murine model with multiple features of atopic dermatitis: structural, immunolog- ic, and biochemical changes following single versus multiple oxazolone challen- ges[ J]. J Invest Dermatol, 2008, 128 (1):79-86.
  • 10Bae IH, Yun JW, Seo JA, et al. Immu- nohistological comparison of cutaneous pathology of three representative murine atopic dermatitis models [ J ]. J Dermatol Sci, 2010, 59(1) :57 -60.

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