摘要
目的:研究CD26+表型卵巢癌SKOV-3细胞体外侵袭能力。方法:利用免疫荧光标记物流式分选法(FACS)分选SKOV-3细胞系中CD26+表型细胞,并利用transwell小室进行体外侵袭实验检测CD26+及CD26-表型细胞侵袭能力。结果:SKOV-3细胞系中存在CD26+表型细胞,占7.3%,CD26-细胞占92.7%;CD26+细胞侵袭过人工基底膜细胞数为111.5±7.27,CD26-细胞侵袭过人工基底膜细胞数为60.2±5.53,CD26+亚群细胞穿过人工基底膜细胞数强于CD26-细胞亚群,差异有统计学意义(P<0.05)。结论:SKOV-3细胞系中存在CD26+表型细胞,且CD26+表型细胞侵袭能力强于CD26-细胞,提示CD26可能作为卵巢癌干细胞标志物并成为卵巢癌抗转移的新靶点。
Objective： To research the invasive ability of CD26 ＋ phenotype ovarian cancer SKOV -3 cell line in vitro. Methods： FACS was used to dissociate CD26 ＋ pbenotype ovarian cancer SKOV - 3 cell line ; the invasive abilities of CD26 ＋ and CD26 - phenotypes o- varian cancer SKOV -3 cell lines were detected by in vitro invasive assay with transwell. Results： CD26 ＋ phenotype cells accounted for 7.3% of ovarian cancer SKOV - 3 cells, and CD26 - phenotype cells accounted for 92. 7% ; CD26 ＋ phenotype ovarian cancer SKOV - 3 cells invaded （ 11 l. 5 ± 7.27 ） artificial basilar membrane cells, while CD26 - phenotype ovarian cancer SKOV - 3 cells invaded （ 60. 2 ± 5.53 ） artificial basilar membrane ceils ; the former was statistically significantly higher than the latter （ P 〈 0. 05 ） . Conclusion： CD26 ＋ phenotype ceils exist in ovarian cancer SKOV -3 cell line, and the invasive ability of CD26 ＋ phenotype ovarian cancer SKOV -3 cells is stronger than that of CD26 - phenotype ovarian cancer SKOV - 3 cells, which indicates that CD26 maybe one of ovarian cancer stem cell markers and new targets of anti- metastasis of ovarian cancer.
出处
《中国妇幼保健》
CAS
北大核心
2013年第32期5350-5352,共3页
Maternal and Child Health Care of China
基金
河南省杰出青年基金资助〔104100510007〕
