摘要
目的:观察中药提取物三氧化二砷(Arsenic Trioxide,As2O3)对海马神经元Kv4.2、Kv4.3钾通道表达的影响。方法:将SD大鼠随机分为5组,分别经侧脑室注射生理盐水、不同剂量的As2O3(1mg/mL,0.1mg/mL,0.01mg/mL)和4-氨基吡啶(4-AP,15mmol/L),观察给药后大鼠行为学的改变及Western-blot法检测海马神经元Kv4.2、Kv4.3钾通道的表达。结果:As2O3随浓度增加,能够剂量依赖性的引起海马神经元Kv4.2、Kv4.3蛋白表达增强,1mg/mL和0.1mg/mL的As2O3可引起大鼠癫痫样的抽搐发作。结论:As2O3能够影响海马神经元Kv4.2、Kv4.3钾通道的表达并引起癫痫样发作的行为学的改变。
Objective：To observe the effect of arsenic trioxide （As2O3） on expression of Kv4.2 and Kv4.3 of hippocampus neurons.Methods：The SD rats were divided into five groups randomly,and they were given normal saline,As2 O3 （1 mg/mL,0.1 mg/mL,0.01 mg/mL） and 4-amion pyridine （4-AP,15mmol/L）,respectively.Then the behavioral changes were observed in the rats and expressions of Kv4.2 and Kv4.3 of the hippocampus neurons were observed by western-blot.Results：As2O3 enhanced the expression of Kv4.2 and Kv4.3 of the hippocampus neurons dose dependently with the increase of concentration.As2O3 induced epileptiform seizures of the rats at concentrations of lmg/mL and 0.lmg/mL.Conclusion：As2O3 could affect the expressions of Kv4.2 and Kv4.3 of the hippocampus neurons and induce epileptiform behavioral changes.
出处
《中医药学报》
CAS
2013年第5期66-69,共4页
Acta Chinese Medicine and Pharmacology
基金
黑龙江卫生厅课题项目(12531305)
黑龙江省卫生厅课题项目(2012-520)
黑龙江省博士后启动基金项目(101850)
哈尔滨医科大学第一附属医院院基金项目(2009B17)
关键词
三氧化二砷
海马神经元
钾通道
侧脑室注射
Arsenic trioxide
Hippocampus neurons
Potassium channel
Lateral cerebral ventricle injection
