摘要
【目的】评价套式PCR检测试剂盒在疟疾现场监测中的效果。【方法】对疟疾从高度流行区转入低度流行区的非洲科摩罗Moheli岛居民,采集其指血2 098人份,同时制作厚血片和抗凝血样,用自制的套式PCR检测试剂盒检测所采集血样中的疟原虫,并与镜检法进行比较。为了避免假阳性,试验中设置了阴性对照组和阳性对照组。【结果】2 098份血样中,套式PCR技术阳性数140人,阳性率为6.67%;镜检阳性数63人,阳性率为3.00%。【结论】套式PCR检测试剂盒检测疟疾感染具高度敏感性,在疟疾控制转入清除阶段,采用此法普查可发现低密度疟原虫携带者,对快速清除传染源将有重要价值。
Objective To evaluate the nested PCR detection kit as a monitoring tool in the field for detecting positive malaria cases in a malaria elimination program. Methods Blood samples were randomly collected from 2098 residents living in a previously high-endemic, but now low malaria-endemic area, which involved in an antimalarial prograum. A thick blood smear and an anti-coagulated blood sample were prepared from each collected specimen. Results of the nested PCR detection kit method were compared with those of the standard microscopy method for each specimen. To avoid the false positive results, both negative control group and positive control group were set up. Results In 2098 samples, the number of positive results for PCR was 140 （ 6.67% ） and that for microscopy was 63 （ 3.00% ）. Conclusion These results demonstrate that the nested PCR detection kit used in a general survey of low-density plasmodium carrier is more sensitive than routine microscopy, and would be of value in detecting positive cases that would need treatment in a malaria eradication program.
出处
《广州中医药大学学报》
CAS
北大核心
2013年第6期839-842,848,共5页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家科技部项目(编号:2010DFB30500)
国家科学技术部国际合作司项目(编号:KY-2011-013-4)
