摘要
目的 :测定中药升麻中阿魏酸和异阿魏酸的含量。方法 :反相高效液相色谱法。HypersilODS分析柱(2 5 0mm× 4 6mm ,5 μm) ,流动相 :甲醇 -水 -磷酸 (5 0∶15 0∶0 1) ,紫外检测波长 :32 0nm ,灵敏度 :1 0AUFS ,柱温 :2 4℃ ,流速 :1mL·min-1。结果 :阿魏酸在 0 0 42～ 2 10 μg (r =0 9993) ,异阿魏酸在 0 0 43～ 2 14μg (r =0 9994)范围内呈线性 ,阿魏酸和异阿魏酸平均回收率 (n =5 )分别为 98 76 % (RSD =1 6 % )和 98 32 % (RSD =1 8% )。结论 :本方法简便、精确、可靠。
Objectives:To determine ferulic acid and isoferulic acid in Rhizoma Cimicifugae.Methods:The chromatographic conditions included Hypersil ODS column (250 mm×4 6 mm,5 μm) and the mobile phase consisting of a mixture of methanol-water-phosphoric acid (50∶150∶0 1).Detection wavelength was at 320 nm.The flow rate was 1 0 mL·min -1 ,column temperature was at 24 ℃.Results:The calibration curve of ferulic acid was 0 042～2 10 μg ( r= 0 999 3).The calibration curve of isoferulic acid was 0\^043～2 14 μg( r= 0 999 4 ).The average recovery ( n=5 ) of ferulic acid and isoferulic acid were 98 76%( RSD= 1 6%) and 98\^32%( RSD =1 8%) respectively.Conclusion:The method is simple,accurate and reliable.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2000年第6期396-398,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
升麻
阿魏酸
异阿魏酸
高效液相色谱法
测定
Cimicifuga foetida, C.dahurica, C.heracleifolia, ferulic acid,isoferulic acid,HPLC
