摘要
利用与烟草赤星病抗性连锁的6对SSR标记和随机选取的21对SSR标记,对33份具有不同赤星病抗性的烟草种质资源的遗传关系进行了分析。结果表明:基于3个烟草赤星病抗性QTL两侧的6个SSR标记可以较好地将抗病品种和感病品种区分开来。在遗传相似系数(GS)为0.44的水平上,可将33个烟草品种分为4个类群:具有净叶黄(国内)和Beinhart-1000(美国)赤星病抗源的烟草品种聚为一类(Ⅰ类);抗赤星病的CV系列烟草品种聚为一类(Ⅱ类);长脖黄、G140、NC82等感病品种聚为一类(Ⅲ类);香甜烟草(N.suaveolens)单独聚为一类(Ⅳ类)。基于随机选取的SSR标记的聚类结果则不能看出与赤星病抗性的联系。
The phylogenesis of thirty-three tobacco germplasms belonging to different brown spot resistance analyzed by six SSR markers which linked to the tobacco brown spot resistant genes and twenty-one random SSR markers were reported in this study.The results of cluster analysis based on the six tobacco brown spot resistant linked SSR markers flanking the three tobacco brown spot resistant QTLs showed that thirty-three tobacco varieties could be clustered into four groups at a relative low level of GS （genetic similarity coefficient） 0.44.The tobacco varieties which had the resistant resource to tobacco brown spot from the Jingyehuang （China） and Beinhart-1000 （USA） were clustered into group Ⅰ;the tobaccos which belong to CV series and are resistant to tobacco brown spot were clustered into group Ⅱ;N.suaveolens belonging to wild tobacco was in an unique group Ⅳ;while the left which belong to susceptible varieties such as Changbohuang,G140,and NC82 were clustered into group Ⅲ.The results of cluster analysis showed that the SSR markers linked to the tobacco brown spot resistant genes （flanking the resistant QTLs） could cluster the species into resistant and susceptive while the random SSR makers could not.
出处
《西北植物学报》
CAS
CSCD
北大核心
2013年第10期1972-1980,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
云南省科技厅项目(2012BB006)
中国烟草总公司项目(110201002001)
中国烟草总公司云南省公司项目(2010YN02
2011YN06)
关键词
烟草
SSR标记
赤星病
聚类分析
tobaccos
SSR markers
brown spot
cluster analysis
