摘要
以河南温县铁棍山药带腋芽的茎段为外植体进行铁棍山药种苗快繁及珠芽离体再生体系的研究。结果表明：（1）75%乙醇浸泡30 s和5% NaClO消毒15 min配合使用灭菌效果最好;腋芽诱导最适培养基为MS＋0.5 mg·L-1 6-BA＋0.1 mg·L-1 NAA,培养20 d后诱导的多芽体倍数最高,为2.22,高度最高为3.3 cm;继代增殖最适培养基为MS＋1.5 mg·L-1 6-BA,增殖倍数可达4.1;生根培养最适培养基为1/2MS＋0.2 mg·L-1 6-BA＋1.0 mg·L-1 NAA＋0.02%活性炭,平均生根天数为12 d,生根率达100%,根系最长为1.04 cm。（2）用单芽带外植体的接种方式,其珠芽诱导率及诱导的珠芽数显著高于只接单芽的接种方式,珠芽诱导率达88.9%,平均珠芽数为1.50,大小为0.38 cm×0.54 cm;蔗糖浓度为1%～3%有利于珠芽诱导,珠芽整齐度好,形状规则,试管苗叶色浓绿;离体珠芽芽诱导的最适培养基为MS＋1.5 mg·L-1 6-BA＋0.2 mg·L-1 NAA,珠芽在18～22 d发芽,30 d后诱导率最高为83.3%。该实验结果为铁棍山药试管苗的工厂化生产奠定了技术基础。
With the stem segments as explants,we studied the technique about the tissue culture for rapid propagation and in vitro bulbils regeneration system of Dioscorea opposita Thunb from Wen County,Henan Province.The results showed that：（1）The disinfection efficacy which combing 70% alcohol 30 s with 5% NaClO 15 min was the best.The optimal axillary buds induction medium was MS＋0.5 mg·L-1 6-BA＋0.1 mg·L-1 NAA with the maximum multiple buds ratio and buds height.After 20 d,the multiple buds ratio were 2.22 and buds height were 3.3 cm.The optimal subculture medium was MS＋1.5 mg·L-1 6-BA and the propagation coefficient could reach 4.1.The optimal rooting medium was 1/2MS＋0.2 mg·L-1 6-BA＋1.0 mg·L-1 NAA＋0.02% activated carbon,with 12 d for the average rooting day,100% rooting rate and 1.04 cm root length.（2）The cultural method of the explants with single bud,was significantly （P〈0.05） higher than the cultural method of the single bud in the inductivity and average number of bulbils,which the inductivity,average number and size were 88.9%,1.50 and 0.38 cm×0.54 cm,respectively.1%~3% sucrose concentration was helpful to inducing the bulbils with had regular shape and the colour of the plantlets leaves were dark green.The optimal bulbils germination medium was MS＋1.5 mg·L-1 6-BA＋0.2 mg·L-1 NAA.The bulbils germinated at 18~22 d and inductivity reached 83.3% at 30 d.These results had a great potential application value for test-tube plantlet factory production of D.opposita Thunb.
出处
《西北植物学报》
CAS
CSCD
北大核心
2013年第10期2120-2125,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
江苏省农业科技自主创新资金[cx(13)2009]
关键词
铁棍山药
组织培养
腋芽诱导
珠芽诱导
再生体系
快繁
Dioscorea opposita Thunb
tissue culture
the axillary buds induction
bulbils induction
regeneration system
rapid propagation
