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胸水中干扰素释放测定在结核性胸膜炎诊断中的价值 被引量:8

Value of interferon-Y release assay of pleural effusion in the diagnosis of tuberculous pleuritis
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摘要 目的探讨胸水中体外诱发的y-干扰素释放测定(IGRA)在结核性胸膜炎诊断中的价值。方法收集本院2009年11月至2012年1月呼吸内科连续收治的62例病理确诊的结核性胸膜炎患者和30例其他原因如肿瘤、炎性反应的胸膜炎患者,治疗前两周内收集胸水,结核分枝杆菌早期分泌抗原靶6(ESAT-6)刺激后应用ELISA法进行y-干扰素浓度测定。结果结核性胸膜炎患者胸水经ESAT-6抗原刺激后,y-干扰素增加的百分率为16%-53%,IGRA的阳性率为90.3%(56/62);非结核性胸水患者经ESAT-6抗原刺激后,y-干扰素增加6%-8%,30例中有2例IGRA为临界状态,其余均为阴性。结核性胸膜炎患者胸水1.干扰素基础值水平也高于非结核性胸水患者[(1825±69)ng/L比(967±105)ng/L,P〈0.05]。结论胸水IGRA检测有望成为鉴别结核性胸膜炎的一种准确可靠的方法。 Objective To explore the value of interferon-Y, (IFN-Y) release assay (IGRA) of pleural effusion in the diagnosis of tuberculous pleuritis. Methods Between November 2009 and January 2012, 62 patients with pathologically confirmed diagnosis of tuberculous pleuritis and 30 with miscellaneous diseases (i. e. tumor and inflammatory pleuritis) from the Department of Respiratory Internal Medicine, Henan Provincial Chest Hospital were enrolled in the study. The pleural effusion was sampled 2 weeks prior to the treatment. IFN-Y concentration was measured by enzyme- linked immunosorbent assay following stimulation with early secretory antigen target-6 (EAST-6) for Mycobacterium tuberculosis. Results Positire rate of IGRA was 90.3% (56/62) in tuberculous pleuritis patients, with an increase by 16%-53% in IFN-Y production. Though patients with non-tuberculous pleuritis yielded a 6%-8% increment in IFN-Y release following EAST-6 stimulation, 30 cases tested negatively to IGRA with exception of 2 with borderline positive results. Pleuralfluid of tuberculous pleuritis patients was characterized by a higher level of IFN-Y than that of other patients [ (1825 ± 69)ng/L vs (967 ± 105)ng/L, P〈0.05 ]. Conclusion Pleural fluid IGRA is a potentially accurate and reliable approach for differential diagnosis of tuberculous pleuritis.
出处 《中华生物医学工程杂志》 CAS 2013年第4期324-326,共3页 Chinese Journal of Biomedical Engineering
基金 河南省医学科技攻关计划项目(201003136、201001014)
关键词 结核 胸膜 Y干扰素释放测定 胸水 诊断 Tuberculosis, pleural Interferon-Y/release assay Hydrothorax Diagnosis
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参考文献8

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二级参考文献7

  • 1谢莉,马玙,高微微,李传友,张宗德,陈效友,古书香,邢爱英,郑素华,刘忠泉,陈曦,韩喜琴.抗原刺激后外周血单个核细胞γ干扰素释放反应在结核分枝杆菌感染和结核病诊断中的意义[J].中华结核和呼吸杂志,2005,28(8):545-549. 被引量:13
  • 2石瑞如,张国龙.抗原诱发的γ-干扰素测定[J].中国防痨杂志,2006,28(5):317-320. 被引量:5
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