摘要
目的:建立金莲花药材高效液相色谱(HPLC)指纹特征图谱的方法。方法:采用Phenomenex Gemini C18柱(4.6 mm×250 mm,5μm)、乙腈-0.1%磷酸为流动相梯度洗脱,流速1.0 mL/min,柱温35℃,检测波长230 nm,用国家药典委员会颁布的中药色谱指纹图谱相似度评价系统(2004A版)进行相似度评价。结果:建立金莲花药材指纹图谱,确定31个共有峰,指认了5个色谱峰,金莲花各药材指纹图谱与共有模式相似度均在0.85以上。结论:HPLC指纹图谱为金莲花药材质量的更有效控制提供了依据。
Objective： To establish HPLC fingerprint analysis method of Trollius chinensis. Methods： The HPLC method was carried out on Phenomenex Gemini C18 （4.6 mm ×250 mm, 5μm） using aeetonitrile-0.1% phosphoric acid as mobile phase. The flow rate was 1.0 mL/min. The column tem- perature was 35 ℃. Detection wavelength was 230 nm. The similarity was evaluated with similarity evaluation system for chromatographic fingerprint of tradition Chinese medicine （2004A） issued by state pharmacopoeia committee. Results： HPLC fingerprint of Trollius chinensis was established. 31 common peaks were identified in the fingerprint and 5 chromatographic peaks were obtained. The similarity between the mutual mode and each Trollius chinensis fingerprint was more than 0.85. Conclusions： The HPLC fingerprint provides basis for the effective quality control of Trollius chinensis.
出处
《贵阳医学院学报》
CAS
2013年第5期489-492,共4页
Journal of Guiyang Medical College
基金
贵阳市云岩区科技局[2010]第14号
贵州省科技厅中药现代化[2011]5081号
关键词
金莲花
色谱法
高压液相
指纹图谱
相似度
Trollius chinensis
chromatography, high pressure liquid
fingerprint
similarity
