摘要
目的建立食品中酸性橙Ⅱ、碱性橙2和碱性嫩黄测定的超高效液相色谱-串联质谱法。方法采用C18(2.1 mm×50 mm,1.7μm)色谱柱,以乙腈和0.1%甲酸的5 mmol/L乙酸铵水溶液为流动相,梯度洗脱,流速为0.3 ml/min,柱温40℃,碱性橙2、碱性嫩黄,ESI(+);酸性橙Ⅱ,ESI(-),在此色谱质谱条件下进行检测。结果该方法各组分检出限:酸性橙Ⅱ7.0μg/kg;碱性橙2 5.0μg/kg;碱性嫩黄5.0μg/kg,线性范围:酸性橙Ⅱ为7.0μg/kg^500.0μg/kg;碱性橙2和碱性嫩黄为5.0μg/kg^500.0μg/kg。加标回收率:酸性橙Ⅱ为93.5%~111.0%,;碱性橙2为93.5%~109.0%;碱性嫩黄为89.5%~108.0%。结论该方法操作简便,准确,快速,提高了样品检出灵敏度,能够同时检测3种非食用色素的含量。
Objective To establish a UPLC - MS/MS method for determination of acid orange II , basic orange 2 and auramine O in foods. Methods Acid orange II, basic orange 2 and auramine O were separated on a C18 column using acetonitrile： ammonium acetate as the mobile phase at a flow rate of 0.3 ml/min, with the column temperature at 40 C, Then basic orange 2 and anramine O were detected with ESI （ ＋ ）, while acid orange II was detected with ESI -. Results The detection limits of acid orange II , basic orange 2 and auramine O were 7.0 μg/kg, 2.5 μg/kg and 5.0 μg/kg respectively. The calibration curves were linear in the range of 7.0 μg/kg - 500.0 μg/kg for acid orange II , 5.0 μg/kg - 500.0μg/kg for basic orange 2 and auramine O, while the recovery rates were 93.5% - 111.0%, 93.5% N 109.0% and 89.5% - 108.0%. Conclusion The method is simple, precise, fast and suitable for determination of acid orange II , basic orange 2 and auramine O in foods.
出处
《中国卫生检验杂志》
北大核心
2013年第12期2593-2594,2638,共3页
Chinese Journal of Health Laboratory Technology
关键词
酸性橙Ⅱ
碱性橙2
碱性嫩黄
超高效液相色谱
Acid orange II
Basic orange 2
Auramine O
Ultra performance liquid chromatography
