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Global view of transcriptome in the brains of aged NR2B transgenic mice

Global view of transcriptome in the brains of aged NR2B transgenic mice
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摘要 NR2B subunits are involved in regulating aging, in particular, age-related learning and memory deficits. We examined 19-month-old NR2B transgenic mice and their littermate controls. First, we detected expression of the NR2B subunit gene, Grin2b, in the neocortex of transgenic mice using real-time PCR. Next, we used microarrays to examine differences in neocortical gene expression. Pathway and signal-net analyses identified multiple pathways altered in the transgenic mice, in-cluding the P53, Jak-STAT, Wnt, and Notch pathways, as well as regulation of the actin cytoskeleton and neuroactive ligand-receptor interactions. Further signal-net analysis highlighted the P53 and insulin-like growth factor pathways as key regulatory pathways. Our results provide new insight into understanding the molecular mechanisms of NR2B regulated age-related memory storage, normal organismal aging and age-related disease. NR2B subunits are involved in regulating aging, in particular, age-related learning and memory deficits. We examined 19-month-old NR2B transgenic mice and their littermate controls. First, we detected expression of the NR2B subunit gene, Grin2b, in the neocortex of transgenic mice using real-time PCR. Next, we used microarrays to examine differences in neocortical gene expression. Pathway and signal-net analyses identified multiple pathways altered in the transgenic mice, in-cluding the P53, Jak-STAT, Wnt, and Notch pathways, as well as regulation of the actin cytoskeleton and neuroactive ligand-receptor interactions. Further signal-net analysis highlighted the P53 and insulin-like growth factor pathways as key regulatory pathways. Our results provide new insight into understanding the molecular mechanisms of NR2B regulated age-related memory storage, normal organismal aging and age-related disease.
出处 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第29期2734-2743,共10页 中国神经再生研究(英文版)
基金 supported by the National Natural Science Foundationof China,No.30800312 and 30970368 the Specialized Research Fund for the Doctoral Program of Higher Education,No.20070269026 the Fundamental Research Fund for the Central Universities,No.78210020 Innovation Program of Shanghai Municipal Education Commission,No.10ZZ35
关键词 neural regeneration memorygrowth factor grants-supportedNR2B transgenic mice aging gene expression P53 insulin-likepaper NEUROREGENERATION neural regeneration memorygrowth factor grants-supportedNR2B transgenic mice aging gene expression P53 insulin-likepaper neuroregeneration
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