期刊文献+

SPE-HPLC法同时测定安神补脑液中二苯乙烯苷和淫羊藿苷的含量 被引量:6

Simultaneous Determination of Stilbeneglycoside and Icariin in Anshen Bunao Liquids by SPE-HPLC
原文传递
导出
摘要 目的:建立安神补脑液中二苯乙烯苷和淫羊藿苷的含量测定方法。方法:以XTerra RP C_(18)色谱柱为分离柱,以甲醇-水(60:40)为流动相,检测波长:320nm;柱温:30℃;流速:1.0ml·min^(-1);进样量:10μl。结果:二苯乙烯苷的线性范围为10.62~127.44μg·ml^(-1),r=0.9996,平均回收率为98.26%,RSD=1.01%(n=6);淫羊藿苷的线性范围为0.82~9.89μg·ml^(-1),r=0.9997,平均回收率为98.59%,RSD=1.33%(n=6)。结论:该方法简便、快速、准确,可用于安神补脑液中二苯乙烯苷和淫羊藿苷的含量测定。 Objective: To establish a method to determine stilbeneglycoside and icariin in Anshen Bunao liquids. Method: SPE- HPLC was conducted on an XTerra RP C18 column with the mobile phase of methanol-water(60: 40). Result: The linear range of stil- heneglycoside was 10. 62-127. 44 μg · ml^-1 (r =0.999 6), and the average recovery was 98.26% (RSD = 1.01% ,n =6). The linear range of icariin was 0.82-9.89 μg · ml^-1 ( r = 0.999 7 ) , and the average recovery was 98.59% ( RSD = 1.33% , n = 6 ). Conclusion: The method is simple and can be used in the determination of stilbeneglycoside and icariin in anshen bunao liquids.
出处 《中国药师》 CAS 2013年第10期1493-1494,共2页 China Pharmacist
关键词 固相萃取-高效液相色谱法 二苯乙烯苷 淫羊藿苷 安神补脑液 含量测定 SPE- HPLC Stilbeneglycoside Icariin Anshen Bunao liquids Determination
  • 相关文献

参考文献3

二级参考文献4

共引文献876

同被引文献102

引证文献6

二级引证文献18

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部