摘要
目的:建立潍坊地区鲜无蹼壁虎地道药材的鉴别方法。方法:夏秋季捕捉潍坊地区成体鲜壁虎,随机抽恥100只,断食72h,水清洗晾干,95%乙醇中固定,电子天平称重,游标卡尺测长度。观察尾基部的肛疣数目特征、鼻间鳞和腹部环体一周的鳞片数目特征、指趾腹面的鳞片形状特征和指趾末端结构特征。利用十二烷基硫酸钠聚丙烯酰胺凝胶电泳技术(SDS-PACE),观察无蹼壁虎及其伪品蜥蜴的电泳迁移率,根据其蛋白电泳区带的差异。绘制电泳图谱。结果:潍坊地区无蹼壁虎头部扁平较大、呈三角形、躯干背部疣鳞极少,两侧几乎无疣鳞,大形鼻间鳞1~2枚。腹部最宽处的一横列鳞22~28枚。足指间无蹼,每指的指腹和指尖足底侧有攀瓣,一足或多足足部有暗红色斑点,分布在足背上,95%乙醇浸泡足部暗红色斑点消失。经试验鉴别,无蹼壁虎与蜥蜴的电泳图谱有显著差异,各有其特有谱带。结论:潍坊地区壁虎均为无蹼壁虎。无蹼壁虎平均体质量为(3.7±0.46)g,体长为(11.08±1.18)cm。无蹼壁虎与蜥蜴的凝胶电泳区带具有显著区别,可作为有效鉴别依据之一。
Objective： To establish identification method for fresh G. swinhonis Guenther in Weifang district. Method： Totally 100 fresh Geckoes were captured randomly in summer and autumn. After fasted for 72h and cleaned with water, the Geckoes were body weighted using an electronic balance, and the body length was measured by a sliding caliper. The number characteristics of mamilla le- pis on the tail base, internasal scales and scales around the belly were observed, and the structure properties of scales on the digit ven- ter and end were also studied. SDS-PAGE was used to detect the electrophoretie mobility shift of G. swinhonis Guenther and lizard, and the electrophoregram was respectively drawn according to the difference in the protein electrophoresis. Result： G. swinhonis Guenther in Weifang district has a fiat triangle head, trunk back with few while two sides without mamilla lepis, 1-2 big lepis on the nose, 22-28 rank lepis on the broadest belly, foot and interdigit without web while kermesinus spot on the polypodia back. The electrophoregram re- vealed significant difference between G. swinhonis Guenther and lizard with characteristic bands. Conclusion： The Geckoes in Weifang district are all G. swinhonis Guenther with the mean body weight of （ 3.7 ± 0.46） g and body length of （ 11.08 ± 1.18 ） cm. SDS-PAGE can be used to distinguish G. swinhonis Guenther and lizard. The fingerprint method is simple and reliable, and can be applied in the i- dentification.
出处
《中国药师》
CAS
2013年第10期1521-1524,共4页
China Pharmacist
基金
国家自然科学基金项目(编号:30572323)
关键词
鲜无蹼壁虎
鉴定
凝胶鉴别
潍坊地区
Fresh G. swinhonis G^nther
Identification
SDS-PAGE
Weifang district
