摘要
目的:建立测定小儿肺热咳喘口服液中绿原酸含量的方法。方法:采用高效液相色谱法,色谱柱:Agilent Eclipse XDB-C_(18)(150mm×4.6mm,5μm);流动相:甲醇-0.5%冰醋酸溶液(20:80);检测波长:327 nm;流速:1.0ml·min^(-1);柱温:室温;进样量:10μl。结果:回归方程为Y=29.96X+1.684,绿原酸在2.184~218.4g·ml^(-1)范围內与其峰面积线性关系良好(r=0.9999),平均回收率为100.90%,RSD为1.62%。结论:本方法快速、专属性强、重复性好、结果准确可靠,可用于测定小儿肺热咳喘口服液中绿原酸的含量。
Objective ： To establish an HPLC method for the determination of chlorogenic acid in Xiao＇ er Feire Kechnan oral solu- tions. Method： An Agilent Eclipse XDB-C18 （4.6 mm× 150 mm, 5 μm ） column was used, the flow rate was 1.0 ml · min ^-1, the mobile phase consisted of methanol-0.5% glacial acetic acid （20： 80） with the detection wavelength at 327 nm, the column tempera- ture was at the room temperature and the injection volume was 10 μl. Result： The regression equation was Y = 29.96X ＋ 1. 684,the linear range was 2. 184-218.4 g · ml^-1 （ r = 0,999 9） with the average recovery of 101.01% （ RSD = 2.35% ）. Conclusion： The method is rapid, simple and accurate with good specificity and reproducibility, and it can be used in the content determination of ch- borogenic acid in Xiao＇ er Feire Keehuan oral solutions.
出处
《中国药师》
CAS
2013年第10期1592-1593,共2页
China Pharmacist
