摘要
目的：建立白头翁中白头翁皂苷D的含量测定方法.方法：采用HPLC测定白头翁皂苷D的含量,色谱条件为依利特Hypersil ODS2C18色谱柱（4.6 mm ×250 mm,5μm）,流动相乙腈-水-甲酸（38∶62∶0.1）,流速1.0 mL·min-1,检测波长203 nm,柱温25℃,考察不同产地白头翁中白头翁皂苷D的含量差异.结果：白头翁皂苷D线性范围0.3112～3.112 μg（r =0.9999）,精密度RSD 1.36％,重复性RSD 1.65％,供试品溶液在24 h内稳定,平均加样回收率98.07％（RSD 0.64％）.吉林产白头翁中白头翁皂苷D含量最高（0.256％）,比广东产白头翁高了约20倍.结论：该测定方法简便易行、结果准确、重复性好.不同产地的白头翁中白头翁皂苷D的含量差异较大,选用时应慎重.
Objective：To establish a determination method for pulsatilla saponin D in Pulsatillae Radix.Method：The content of pulsatilla saponin D was determined by RP-HPLC,chromatographic conditions were as follows：Elite Hypersil ODS2 C18 column （4.6 mm × 250 mm,5 μm）,mobile phase of acetonitrile-water-formic acid （38 ∶ 62 ∶ 0.1）,flow rate 1.0 mL · min-1,detection wavelength 203 nm,column temperature 25 ℃.Content difference of pulsatilla saponin D in Pulsatillae Radix from different areas was investigated.Result：Pulsatilla saponin D had good linear relationship in 0.3112-3.112 μg （r =0.9999）,RSD of instrument precision was 1.36％,RSD of method repeatability was 1.65％,samples solution was stable within 24 h,the average recovery was 98.07％ （RSD 0.64％）.The content of pulsatilla saponin D in Pulsatillae Radix from Jilin was the highest （0.256％）,which was 20 folds higher than Pulsatillae Radix from Guangdong.Conclusion：This method was simple,accurate and repeatable.The content of pulsatilla saponin D in Pulsatillae Radix from different areas had much difference,so materials choice should be careful.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第20期36-38,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技重大专项重大新药创制项目(2011ZX11102-001-19)
江西自然科学基金项目(20114BAB215045)
江西省教育厅产学研合作项目(GJJ11006)
关键词
白头翁
白头翁皂苷D
含量测定
三萜皂苷
抗肿瘤作用
Pulsatillae Radix
pulsatilla saponin D
content determination
triterpenoid saponins
anti-tumor effect
