摘要
以木本植物蒙古沙冬青的叶片和根为材料,采用改良Trizol法提取总RNA。结果表明,与传统Trizol法相比,改良Trizol法能有效去除提取过程中残留的异硫氰酸胍、β-巯基乙醇以及植物本身的糖、酚类物质,获得高质量总RNA。改良Trizol法提取总RNA技术已被成功应用于蒙古沙冬青转录组数据库的建立。该方法可为木本植物提取高质量RNA提供借鉴。
Total RNA was extracted using an improved Trizol method, with the leaves and roots of xylophyta, Ammopiptanthus mongolicus （ Maxim ） Cheng f. The result showed that compared to traditional Trizol method, the improved Trizol method can effectively remove residual guanidinium isothiocyanate, beta-mercaptoethanol in extraction process, as well as the sugar and polyphenols of the plant itself, and high-quality total RNA was obtained. The improved Trizol method has been successfully applied to transcriptome database constrction of A. mongolicus. It also provided a reference for xylophyta to extract high-quality RNA.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第10期87-92,共6页
Biotechnology Bulletin
基金
国家自然科学基金项目(31070361)
中央高校基本科研业务费专项基金(1112KYQN31)
中央民族大学"985工程"项目(MUC98504-14
MUC98507-08)
国家大学生创新训练项目(GCCX2013110015)
