摘要
目的探讨Genistein对高侵袭性乳腺癌MDA-MB-231细胞的增殖、侵袭和转移能力的影响。方法采用CCK-8实验和流式细胞技术研究Genistein对乳腺癌MDA-MB-231细胞增殖的影响;细胞侵袭和迁移实验结合Western blot检测Genistein作用前后细胞内基质金属蛋白酶2(MMP-2)和血管内皮生长因子(VEGF)蛋白的变化,研究其对乳腺癌细胞侵袭和迁移能力的影响及可能的作用机制。结果 CCK-8实验结果显示:Genistein处理乳腺癌MDA-MB-231细胞24-h,可明显抑制癌细胞增殖,IC50为18.7μmol/L。流式细胞技术检测细胞周期显示:经相同处理的乳腺癌MDA-MB-231细胞,处于S期的比例显著下降。细胞侵袭实验和迁移实验结果显示:随着Genistein浓度的增加,侵袭和迁移的细胞数量逐渐减少,差异有统计学意义(P<0.05)。Western-blot 结果显示:Genistein处理后可降低MMP-2和VEGF蛋白的表达。结论 Genistein可抑制乳腺癌MDA-MB-231细胞增殖,降低其侵袭和迁移能力,其可能的机制是降低乳腺癌MDA-MB-231细胞MMP-2和VEGF的表达。
Objective To investigate the effects of genistein on proliferation,invasion and metastasis of MDA-MB-231 breast cancer cells.Methods The proliferation of MDA-MB-231 breast cancer cells was measured by CCK-8 assay and flow cytometry assay.Transwell assay and Westem blot assay were used to evaluate the migration and invasion ability of MDA-MB-231 breast cancer cells.Results 24 h after genistein treatment,CCK -8 assay showed that the proliferation of breast cancer cells was inhibited with an IC50 value of 18.7 μmol/L Cell cycle analysis by flow cytometry assay confirmed that the proportion of cells in S phase decreased significantly after genistein treatment （24 h）.The number of migrating cells in transwell assay was sharply reduced.The invasion of MDA-MB-231 cells was inhibited compared with the blank control group （P 〈 0.05）.Westem blot assay revealed that MMP-2 and VEGF decreased significantly by 10 and 20 μmol/L genistein.Conclusion Genistein could effectively inhibit the growth and invasion ability of MDA-MB-231 breast cancer cells in a dose dependent pattem in vitro,and the reduction of MMP-2 and VEGF expression may play an important role in this process.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2013年第10期873-877,共5页
Journal of China Medical University
基金
国家自然科学基金(81272430)
