摘要
应用分子印迹技术建立了乐果残留量的快速定量分析方法.以乐果为模板分子,采用本体聚合法按不同配比制备了乐果分子印迹聚合物,以平衡吸附实验考察其对乐果的特异性吸附能力.结果表明,当n(乐果)∶n(丙烯酰胺)=1∶4时,分子印迹聚合物对乐果的吸附效果最佳.采用分子印迹聚合物对乐果进行固相萃取,并对分子印迹固相萃取中样品溶剂、洗脱溶剂进行了优化选择.结果表明,当THF(5%乙酸)作为洗脱剂时,洗脱效果最好,洗脱率为98%.以钙黄绿素-Pd2+为荧光探针,采用荧光分光光度法对乐果进行测定.乐果的质量浓度在0.05～0.25mg/L范围内与荧光强度有良好的线性关系(R2=0.998 1).以茶叶样品为基体,用标准加入法作回收试验,测得回收率为92%～105%.
In this paper, a rapid quantitative analysis method was developed for detecting the residues of dimethoate using molecularly imprinted techniques. The molecularly imprinted polymers were synthesized by bulk polymerization, using dimethoate as the template molecule. The binding capacity of the MIP for dimethoate was investigated by equilibrium adsorption experiments. The results showed that MIP exerted the best binding capacity for dimethoate when the proportion of dimethoate, acrylic Amide （AAM） was 1 ： 4. In molecularly imprinted solid phase extraction experiment, the sample solvent and elution solvent were optimized. The results showed that the elution effect could be the best when THF （5% acetic acid）was selected as eluent, the elution rate was 98%. Meantime, calcein-Pd2＋ as a fluorescence probe, fluorescence spectrophotometry was employed to detect the concentration of dimethoate. There was good linear relationship between the magnitude of increase in fluorescence intensity and concentration of dimethoate was kept in the range of 0. 05 - 0. 25 mg/L（R2 = 0. 998 1）. Recovery of the method was tested by standard addition method, and the values of recovery obtained were ranged from 92% to 105%.
出处
《浙江工业大学学报》
CAS
2013年第5期495-499,共5页
Journal of Zhejiang University of Technology
关键词
分子印迹技术
固相萃取
农药残留
荧光比色法
molecularly imprinted .technique
solid phase extraction
pesticide residue
fluorescence spectrophotometry
