摘要
目的:观察20(s)-原人参二醇(PPD)对体外培养宫颈癌Siha细胞生长周期的作用及其对p53、p21及细胞周期素E(cyclin-E)基因转录和表达的影响,探讨PPD抑制Siha细胞增殖的机制。方法:体外培养人宫颈癌Siha细胞,将其分为阴性对照组(乙醇)和实验组(20μg·L-1 PPD),分别用乙醇和PPD处理体外培养的宫颈癌Siha细胞,48h后流式细胞术检测细胞周期,Real time PCR和Western blotting法检测宫颈癌Siha细胞内p53、p21及cyclin-E mRNA及蛋白的表达情况。结果:与阴性对照组比较,20μg·L-1 PPD处理48h后,G0/G1期Siha细胞比例增加(P<0.01),G1期阻滞作用明显增强;Siha细胞中p53与p21mRNA及蛋白表达水平上调(P<0.01),cyclin-E mRNA及蛋白表达水平下调(P<0.01)。结论:PPD可抑制人宫颈癌Siha细胞增殖,其机制可能与上调p53、p21基因表达,下调cyclin-E基因表达有关。
Objective To observe the effect of 20（s）-protopanaxadiol（PPD）on the cell cycle of Siha cells in vitro and to detect the transcription and expressions of p53,p21and cyclin-E,and to clarify the mechanism of its inhibitory effect on proliferation of Siha cells.Methods The Siha cells cultivated in vitro were treated with alcohol（negative control group）and 20μg·L-1 PPD（PPD group）,respectively.The cell cycle was detected by FCM 48h after treatment.The mRNA and protein expressions of p53,p21and cyclin-E in Siha cells were analyzed by Real time PCR and Western blotting.Results Compared with negative control group,after treated with 20μg·L-1 PPD for 48h,the ratio of Siha cells at G0 /G1 phase was increased（P0.01）,the blockage of G1 phase was obviously enhanced;the transcription and protein expressions of p53and p21were up-regulated（P0.01）;while the transcription and expression of cyclin-E were significantly decreased（P0.01）.Conclusion PPD may inhibit the proliferation of Siha cells,and its mechanism may be related to up-regulating the expressions of p53and p21and down-regulating the expression of cyclin-E.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2013年第5期909-912,I0003,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅自然科学基金资助课题(201015195)
