摘要
本文以苹果腐烂病菌为指示菌,采用菌丝生长速率方法测定补骨脂的抑菌活性,其中提取物采用浸渍法提取,通过反复柱层析、薄层层析等分离纯化方法,对补骨脂粗提物的活性成分进行系统的分离,最终得到5种活性物质,经波谱分析法鉴定5种化合物分别为补骨脂酚(bakuchiol)、呋喃香豆素精(bakuchicin)、补骨脂查尔酮(bavachalcone)、补骨脂定(psoralidin)和补骨脂二氢黄酮(bavachin)。经过室内毒力试验,5种化合物的EC50分别为6.117、60.441、3.420、36.815和6.144 mg·L 1,EC90分别为204.480、97.795、15.334、221.860和89.631 mg·L 1,表明5种化合物对苹果腐烂病菌均具有很高的抑制活性。
The inhibitory activity of Psoralea corylifolia L. extracted by immersion method against Valsa mali was tested. The extracts ofP. corylifolia were repeatedly separated and purified by column chromatography, thin layer chromatography and so on, and five compounds were identified as bakuchiol, bavachalcone, bavachin, bakuchicin and psoralidin by spectrum analysis. The antifungal activity of five compounds were tested in vitro and they showed high inhibiting activity to the growth of the hyphae, with the ECs0 values were 6.117, 60.441, 3.420, 36.815, 6.144 mg.L-1 and the EC90 values were 204.480, 97.795, 15.334, 221.860, 89.631 mg.L-1 to V. mali, respectively.
出处
《中国生物防治学报》
CSCD
北大核心
2013年第4期655-660,共6页
Chinese Journal of Biological Control
基金
"十二五"国家科学支撑计划项目(2011BAE06B02)
辽宁省教育厅高等学校科学研究项目(L2010432)
高新技术产业发展与科技攻关计划项目(F12-108-3-00)
关键词
补骨脂
抑菌活性
苹果腐烂病菌
分离
鉴定
波谱分析法
Psoralea corylifolia
antifungal activity
Valsa mali
separation
identification
spectrum analysis
